antibody

Mouse Monoclonal [clone A00121.01] (IgG1) to Human ABCA1

MBS245250

0.05 mg

495 USD

monoclonal

mouse

nonconjugated

A00121.01

human, mouse, chicken

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Mouse Monoclonal [clone A00121.01] (IgG1) to Human ABCA1

ABCA1

Anti-ABCA1 Antibody (aa1800-2260, clone A00121.01) IHC-plus; ABCA1; ABC-1; ABC1; CERP; HDLDT1; Tangier disease; ATP-binding cassette 1; Td; Membrane-bound; TGD; Human ABCA1

ATP-binding cassette sub-family A member 1; ATP-binding cassette sub-family A member 1; ATP-binding cassette sub-family A member 1; membrane-bound; ATP-binding cassette transporter A1; cholesterol efflux regulatory protein; ATP-binding cassette, sub-family A (ABC1), member 1; ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1; Cholesterol efflux regulatory protein

ABCA1

ABCA1; ABCA1; TGD; ABC1; CERP; ABC-1; HDLDT1; ABC1; CERP; ABC-1; ATP-binding cassette 1

ABCA1_HUMAN

Monoclonal

IgG1

A00121.01

Mouse

Chicken, Human, Mouse

2261

Specifically ABCA1 from human, mouse, and chicken.

Immunoaffinity Purified

PBS, pH 7.4, 0.05% sodium azide.

1 mg/ml

Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

ELISA, IHC-P (10 ug/ml), WB. Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.

Target Species: Human. Immunogen Description: Recombinant fragment, corresponding to amino acids 1800-2260 of Human ABCA1.

Immunogen Type: Recombinant protein. Immunogen: ABCA1 antibody was raised against recombinant fragment, corresponding to amino acids 1800-2260 of Human ABCA1. Antigen Modification: aa1800-2260. Usage Summary: Immunohistochemistry: . MBS245250 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2384MBS245250 was determined to be 10 ug/ml.

Family: Transporter. Subfamily: ATP-binding cassette - ABCA/ABC1

This membrane-associated protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extracellular and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDRITAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein functions as a cholesterol efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangiers disease and familial high-density lipoprotein deficiency. ABCA1 (ATP-binding Cassette, Sub-family A, Member 1), also known as ABC1 (ABC Transporter 1) or CERP (Cholesterol efflux regulatory protein), is a member of the family of ATP-binding cassette (ABC) transporters. It is a cAMP-dependent, sulfonylurea-sensitive traffic ATPase, which is located at the plasma membrane and plays a key role in the cellular lipid removal pathway. ABCA1 supports apolipoprotein A-I (APOA 1 )-mediated cellular efflux of cholesterol and cholinephospholipids into the blood circulation; therefore, ABCA 1 defects result in reduced plasma level of high density lipoproteins (HDL) combined with the tissue deposition of cholesteryl esters. Homozygous mutations in ABCA 1 are the cause of Tangier disease, which is characterized by the absence of HDL in plasma, hepatosplenomegaly, peripheral neuropathy, and frequently premature coronary artery disease. Noticeably, heterozygous Tangier disease carriers have an approximately 2-fold reduction in HDL level. More commonly, ABCA1 inherited defects are associated with a milder condition, so-called familial hypoalphalipoproteinemia (FHA). FHA patients, similar to Tangier disease patients, demonstrate a very evident decrease of HDL level; however, they lack clinical manifestations.

21536376

NP_005493.2

NM_005502.3

O95477

ABC Transporters Pathway (83035); ABC Transporters Pathway (436); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Folate Metabolism Pathway (198833); HDL-mediated Lipid Transport Pathway (106158); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135)

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq, Jul 2008]

ABCA1: an ATP-binding cassette (ABC) transporter that regulates cholesterol homeostasis and insulin secretion in beta-cells, and protects against cardiovascular disease. Inactivation of ABCA1 in beta-cells markedly impairs glucose tolerance and insulin secretion in mice. Rosiglitazone, an activator of the peroxisome proliferator-activated receptor-gamma, upregulates ABCA1 in beta-cells. ABCA1 expression in beta-cells is required for the beneficial effects of rosiglitazone on glucose tolerance. Mediates the transport of cholesterol and phospholipids from cells to HDL apolipoproteins, modulating HDL levels and atherogenesis. Induced by bacterial lipopolysaccharides (LPS) and repressed by ZNF202. Phosphorylation on Ser-2054 regulates phospholipid efflux. Unsaturated fatty acids destabilize ABCA1 by activating a PKC-delta pathway that phosphorylates ABCA1 serines. Defects in ABCA1 are a cause of high density lipoprotein deficiencies. Protein type: Membrane protein, multi-pass; Vesicle; Transporter; Membrane protein, integral; Transporter, ABC family. Chromosomal Location of Human Ortholog: 9q31.1. Cellular Component: Golgi apparatus; integral to plasma membrane; perinuclear region of cytoplasm; endocytic vesicle; plasma membrane; phagocytic vesicle; lipid raft; external side of plasma membrane. Molecular Function: apolipoprotein A-I receptor activity; ATPase activity; cholesterol binding; apolipoprotein binding; ATPase binding; apolipoprotein A-I binding; small GTPase binding; protein binding; phospholipid transporter activity; syntaxin binding; cholesterol transporter activity; anion transmembrane transporter activity; phospholipid binding; ATP binding; receptor binding. Biological Process: response to drug; cholesterol metabolic process; regulation of Cdc42 protein signal transduction; intracellular cholesterol transport; cholesterol efflux; lipoprotein metabolic process; cellular lipid metabolic process; endosome transport; phospholipid translocation; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; reverse cholesterol transport; phospholipid efflux; platelet dense granule organization and biogenesis; lysosome organization and biogenesis; response to low density lipoprotein stimulus; positive regulation of cAMP biosynthetic process; protein amino acid lipidation; interleukin-1 beta secretion; phagocytosis, engulfment; response to nutrient; phospholipid homeostasis; peptide secretion. Disease: Hypoalphalipoproteinemia, Primary; Tangier Disease; Hypercholesterolemia, Familial

0.05 mg

495 USD