Mouse Monoclonal (IgG) to Mouse Endostatin | MyBioSource MBS245224 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal (IgG) to Mouse Endostatin

catalog :

MBS245224

quantity :

0.05 mL

price :

495 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS245224

products type :

Antibody

products full name :

Mouse Monoclonal (IgG) to Mouse Endostatin

products short name :

Endostatin

products name syn :

Anti-Endostatin Antibody IHC-plus; COL18A1; Collagen; type XVIII; alpha 1; Collagen alpha-1(XVIII) chain; Endostatin; KNO; KNO1; KS; Multi-functional protein MFP; Antiangiogenic agent; Collagen alpha 1(xviii); Knobloch syndrome; type 1; Mouse Endostatin

other names :

collagen alpha-1(XVIII) chain isoform 1 preproprotein; Collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; endostatin; antiangiogenic agent; multi-functional protein MFP; collagen, type XVIII, alpha 1

products gene name :

COL18A1

other gene names :

COL18A1; COL18A1; KS; KNO; KNO1

uniprot entry name :

COIA1_HUMAN

clonality :

Monoclonal

isotype :

IgG

host :

Mouse

reactivity :

Human, Mouse, Rat

sequence length :

1516

specificity :

Recognizes mouse endostatin, Mr 22kD, an unidentified protein is also weakly detected, Mr 54kD. Detects human protein in IHC-FFPE. It is predicted that the antibody will also detect the rat protein. Cross-reactivity is predicted based on sequence homology. The antibody has not been specifically tested with rat samples at this time.

purity :

Thiophilic adsorption

form :

50 mM sodium phosphate, pH 8.0, 0.05% sodium azide, before the addition of glycerol to 30%. Sourced in Ascites.

storage stability :

Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB)

app notes :

IHC-P (1:100),

other info1 :

Target Species: Mouse. Taget: Endostatin. Immunogen Description: Full-length recombinant murine endostatin.

other info2 :

Immunogen: Endostatin antibody was raised against full-length recombinant murine endostatin. Usage Summary: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS245224 was determined to be 1:100. Western Blot: 1:500-1:2000. Dectects endostatin in mouse liver cell lysates to which 50ng of recombinant human endostatin was added. Mouse liver cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with MBS245224. Proteins were visualized using an IgG (HRP) goat anti-mouse and chemiluminescence detection system.

products categories :

Family: Collagen

products description :

Endostatin, which corresponds to the C-terminal fragment of collagen XVIII, is a potent inhibitor of endothelial cell proliferation, migration and angiogenesis. Recent study shows that endostatin induces tyrosine kinase activity and enhanced apoptosis in FGF-treated endothelial cells. Endostatin is currently being used in Phase I trails as an antitumor agent. Endostatin is the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants.

ncbi gi num :

110611235

ncbi acc num :

NP_085059.2

ncbi gb acc num :

NM_030582.3

uniprot acc num :

P39060

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Degradation Pathway (730309); Collagen Formation Pathway (645288); Degradation Of The Extracellular Matrix Pathway (576263); Direct P53 Effectors Pathway (137939); Extracellular Matrix Organization Pathway (576262); FOXA1 Transcription Factor Network Pathway (137979); Integrin Cell Surface Interactions Pathway (106110)

ncbi summary :

This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

uniprot summary :

COL18A1: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Defects in COL18A1 are a cause of Knobloch syndrome type 1 (KNO1). An autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. Belongs to the multiplexin collagen family. 3 isoforms of the human protein are produced by alternative promoter. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: extracellular matrix; extracellular space; collagen; endoplasmic reticulum lumen; extracellular region; basement membrane. Molecular Function: identical protein binding; protein binding; metal ion binding; structural molecule activity. Biological Process: response to drug; extracellular matrix disassembly; collagen catabolic process; negative regulation of cell proliferation; organ morphogenesis; extracellular matrix organization and biogenesis; visual perception; positive regulation of cell proliferation; endothelial cell morphogenesis; angiogenesis; cell adhesion; response to hydrostatic pressure; positive regulation of cell migration. Disease: Knobloch Syndrome 1

size1 :

0.05 mL

price1 :

495 USD

more info or order :

MyBioSource product webpage