product summary
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company name :
MyBioSource
product type :
antibody
product name :
Mouse Monoclonal [clone 60B1220.1] (IgG1) to Human DNMT / DNMT1
catalog :
MBS245208
quantity :
0.05 mg
price :
495 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
60B1220.1
reactivity :
human, mouse, dog
application :
western blot, immunohistochemistry, immunoprecipitation, chromatin immunoprecipitation
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS245208
products type :
Antibody
products full name :
Mouse Monoclonal [clone 60B1220.1] (IgG1) to Human DNMT / DNMT1
products short name :
DNMT / DNMT1
products name syn :
Anti-DNMT / DNMT1 Antibody (aa637-650, clone 60B1220.1) IHC-plus; DNMT1; AIM; CXXC finger protein 9; CXXC9; DNA methyltransferase 1; DNMT; DNA methyltransferase HsaI; DNA MTase HsaI; HSN1E; M.HsaI; MCMT; Human DNMT; DNMT1
other names :
DNA (cytosine-5)-methyltransferase 1 isoform b; DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; m.HsaI; DNA MTase HsaI; DNA methyltransferase HsaI; CXXC-type zinc finger protein 9; DNA (cytosine-5-)-methyltransferase 1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT
products gene name :
DNMT
products gene name syn :
DNMT1
other gene names :
DNMT1; DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; AIM; CXXC9; DNMT; Dnmt1; DNA MTase HsaI; M.HsaI
uniprot entry name :
DNMT1_HUMAN
clonality :
Monoclonal
isotype :
IgG1
clone :
60B1220.1
host :
Mouse
reactivity :
Bat, Gibbon, Gorilla, Horse, Human, Monkey, Mouse, Rabbit. Predicted Reactivity: Bovine, Dog, Hamster, Pig, Sheep (at least 90% immunogen sequence identity)
sequence length :
1616
specificity :
A synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370). It will cross react with mouse Dnmt1.
purity :
Protein G Purified
form :
PBS, 0.05% BSA, 0.05% sodium azide.
concentration :
0.5 mg/ml
storage stability :
Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
tested application :
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation
app notes :
ChrIP, IHC-P (5 ug/ml), IP, WB (2 - 4 ug/ml). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.
other info1 :
Target Species: Human. Immunogen Description: A synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370). It will cross react with mouse Dnmt1.
other info2 :
Immunogen Type: Synthetic peptide. Immunogen: DNMT / DNMT1 antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370). It will cross react with mouse Dnmt1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Bat, Horse, Rabbit (100%); Sheep, Hamster, Elephant, Dog, Bovine, Pig (93%). Antigen Modification: aa637-650
products categories :
Family: C5-methyltransferase
products description :
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities.
ncbi gi num :
4503351
ncbi acc num :
NP_001370.1
ncbi gb acc num :
NM_001379.2
uniprot acc num :
P26358
ncbi mol weight :
144,465 Da
ncbi pathways :
Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421); DNA Methylation Pathway (1127677); Epigenetic Regulation Of Gene Expression Pathway (1127671); Gene Expression Pathway (105937); Metabolic Pathways (132956); Methionine Degradation Pathway (413353); Methionine Degradation Pathway (468228); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928)
ncbi summary :
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
uniprot summary :
DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described. Protein type: Methyltransferase; EC 2.1.1.37; Methyltransferase, DNA; Cell development/differentiation; Amino Acid Metabolism - cysteine and methionine; Transcription regulation. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: nucleoplasm; centric heterochromatin; replication fork; nucleus. Molecular Function: DNA (cytosine-5-)-methyltransferase activity; protein binding; methyl-CpG binding; DNA binding; zinc ion binding; RNA binding; chromatin binding; transcription factor binding; DNA-methyltransferase activity. Biological Process: negative regulation of histone H3-K9 methylation; maintenance of DNA methylation; transcription, DNA-dependent; gene silencing; chromatin modification; positive regulation of histone H3-K4 methylation; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; regulation of cell proliferation; negative regulation of gene expression, epigenetic; DNA methylation; gene expression; DNA methylation on cytosine. Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie
size1 :
0.05 mg
price1 :
495 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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