Mouse Monoclonal [clone PC10] (IgG2a) to Human PCNA / Cyclin | MyBioSource MBS245134 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal [clone PC10] (IgG2a) to Human PCNA / Cyclin

catalog :

MBS245134

quantity :

0.05 mg

price :

495 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PC10

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, FACS

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS245134

products type :

Antibody

products full name :

Mouse Monoclonal [clone PC10] (IgG2a) to Human PCNA / Cyclin

products short name :

PCNA / Cyclin

products name syn :

Anti-PCNA / Cyclin Antibody (clone PC10) IHC-plus; Cyclin; Human PCNA; Cyclin

other names :

proliferating cell nuclear antigen; Proliferating cell nuclear antigen; proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein; proliferating cell nuclear antigen; Cyclin

products gene name :

PCNA

other gene names :

PCNA; PCNA; ATLD2; PCNA

uniprot entry name :

PCNA_HUMAN

clonality :

Monoclonal

isotype :

IgG2a

clone :

PC10

host :

Mouse

reactivity :

Human, Mouse

sequence length :

261

specificity :

Recombinant rat PCNA

purity :

Affinity Purified

form :

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.

concentration :

0.5 mg/ml

storage stability :

Store at 4 degree C. Do not freeze.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

app notes :

Flo, IHC-P (10 ug/ml), IP, WB. Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.

other info1 :

Target Species: Human. Immunogen Description: Recombinant human PCNA.

other info2 :

Immunogen Type: Recombinant protein. Immunogen: PCNA / Cyclin antibody was raised against recombinant human PCNA.

ncbi gi num :

4505641

ncbi acc num :

NP_002583.1

ncbi gb acc num :

NM_002592.2

uniprot acc num :

P12004

ncbi mol weight :

28,769 Da

ncbi pathways :

BARD1 Signaling Events Pathway (137959); BRCA1-associated Genome Surveillance Complex (BASC) Pathway (413428); BRCA1-associated Genome Surveillance Complex (BASC) Pathway (890555); Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054)

ncbi summary :

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

uniprot summary :

PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase s processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3 - 5 exonuclease and 3 -phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family. Protein type: DNA replication; Cell cycle regulation. Chromosomal Location of Human Ortholog: 20pter-p12. Cellular Component: nucleoplasm; centrosome; PCNA complex; cytoplasm; nuclear replication fork; DNA replication factor C complex; nucleus. Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; purine-specific mismatch base pair DNA N-glycosylase activity; MutLalpha complex binding; receptor tyrosine kinase binding; dinucleotide insertion or deletion binding. Biological Process: mismatch repair; positive regulation of deoxyribonuclease activity; heart development; DNA strand elongation during DNA replication; DNA repair; response to lipid; leading strand elongation; telomere maintenance via semi-conservative replication; G1/S-specific transcription in mitotic cell cycle; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; base-excision repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; telomere maintenance; G1/S transition of mitotic cell cycle; regulation of DNA replication. Disease: Ataxia-telangiectasia-like Disorder 2

size1 :

0.05 mg

price1 :

495 USD

more info or order :

MyBioSource product webpage