antibody

Mouse Monoclonal [clone MEM-259] (IgG1) to Human CD63

MBS245114

0.05 mg

495 USD

monoclonal

mouse

nonconjugated

MEM-259

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS

Antibody

Mouse Monoclonal [clone MEM-259] (IgG1) to Human CD63

CD63

Anti-CD63 Antibody (clone MEM-259) IHC-plus; CD63; CD63 molecule; LAMP-3; ME491; Granulophysin; MLA1; Tetraspanin-30; Tspan-30; CD63 antigen; OMA81H; TSPAN30; Human CD63

CD63 antigen isoform A; CD63 antigen; CD63 antigen; tspan-30; granulophysin; tetraspanin-30; melanoma-associated antigen MLA1; CD63 antigen (melanoma 1 antigen); melanoma-associated antigen ME491; ocular melanoma-associated antigen; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; CD63 molecule; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD_antigen: CD63

CD63

CD63; CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30; MLA1; TSPAN30; LAMP-3; Tspan-30

CD63_HUMAN

Monoclonal

IgG1

MEM-259

Mouse

Human

238

HPB-ALL T cell line

Protein A Purified

PBS, 15 mM sodium azide, approx., pH 7.4.

1 mg/ml

Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

Flo, ICC, IHC-P (10 ug/ml), IP, WB. Usage: Flow Cytometry: Recommended dilution - 2 µg/ml, Positive Controls - detection of activated platelets, neutrophils and basophils. Western Blotting: Application note - Non-reducing conditions. Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for was determined to be 10 ug/ml.

Target Species: Human. Immunogen Description: Cells.

Immunogen: CD63 antibody was raised against cells.

Family: Tetraspan

4502679

NP_001771.1

NM_001780.5

P08962

17,271 Da

Hemostasis Pathway 106028!!Lysosome Pathway 99052!!Lysosome Pathway 96865!!Platelet Activation, Signaling And Aggregation Pathway 106034!!Platelet Degranulation Pathway 106050!!Proteoglycans In Cancer Pathway 782000!!Proteoglycans In Cancer Pathway 782054!!Response To Elevated Platelet Cytosolic Ca2+ Pathway 106048

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

0.05 mg

495 USD