Mouse Monoclonal [clone 9G3.23] (IgG) to Human HEC1 / NDC80 | MyBioSource MBS245080 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal [clone 9G3.23] (IgG) to Human HEC1 / NDC80

catalog :

MBS245080

quantity :

0.05 mL

price :

530 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[9G3.23]

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-NDC80 / HEC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

product information

catalog number :

MBS245080

products type :

Antibody

products full name :

Mouse Monoclonal [clone 9G3.23] (IgG) to Human HEC1 / NDC80

products short name :

[HEC1 / NDC80]

products name syn :

[Anti-HEC1 / NDC80 Antibody (aa56-642, clone 9G3.23) IHC-plus; NDC80; HEC1; Kinetochore protein Hec1; Kinetochore associated 2; HEC; HsHec1; HsNDC80; KNTC2; TID3; Human HEC1; NDC80]

other names :

[kinetochore protein NDC80 homolog; Kinetochore protein NDC80 homolog; kinetochore protein NDC80 homolog; kinetochore associated 2; kinetochore protein Hec1; kinetochore-associated protein 2; highly expressed in cancer protein; retinoblastoma-associated protein HEC; NDC80 kinetochore complex component homolog; NDC80 homolog, kinetochore complex component; highly expressed in cancer, rich in leucine heptad repeats; NDC80 kinetochore complex component; Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC]

products gene name :

[HEC1]

products gene name syn :

[NDC80]

other gene names :

[NDC80; NDC80; HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80; HEC; HEC1; KNTC2; HsHec1]

uniprot entry name :

NDC80_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

[9G3.23]

host :

Mouse

reactivity :

Human

sequence length :

642

specificity :

Human HEC1 protein consisting of amino acids 56-642.

purity :

Protein G Purified

form :

PBS.

concentration :

0.27 mg/ml

storage stability :

+4 degree C or -20 degree C, Avoid repeated freezing and thawing.

tested application :

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP)

app notes :

IHC - Paraffin (1:50). Immunofluorescence. Western blot (1:500 - 1:3000). Immunoprecipitation. Usage Summary: Immunohistochemistry: MBS245080 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for MBS245080 was determined to be 1:50.

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Target Species: Human. Immunogen Description: Recombinant human NDC80 / HEC1. Antigen: Recombinant Protein.

other info2 :

Immunogen Type: Recombinant protein. Immunogen: HEC1 / NDC80 antibody was raised against recombinant human NDC80 / HEC1. Antigen Modification: aa56-642. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products description :

Usage Summary: Immunohistochemistry: MBS245080 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for MBS245080 was determined to be 1:50.

ncbi gi num :

5174457

ncbi acc num :

NP_006092.1

ncbi gb acc num :

NM_006101.2

uniprot acc num :

O14777

ncbi mol weight :

73,913 Da

ncbi pathways :

Aurora B Signaling Pathway (138080); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); M Phase Pathway (105812); Mitotic Anaphase Pathway (105818); Mitotic Metaphase And Anaphase Pathway (730317); Mitotic Prometaphase Pathway (105815); PLK1 Signaling Events Pathway (138007); Resolution Of Sister Chromatid Cohesion Pathway (730315); Separation Of Sister Chromatids Pathway (730318)

ncbi summary :

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

uniprot summary :

HEC1: plays an essential role in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G2/M phase. Phosphorylation by Nek2 is essential for faithful chromosome segregation. Required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores. Protein type: Cell cycle regulation. Chromosomal Location of Human Ortholog: 18p11.32. Cellular Component: kinetochore; membrane; nucleus; cytosol; chromosome, pericentric region. Molecular Function: protein binding; structural constituent of cytoskeleton. Biological Process: mitosis; mitotic spindle organization and biogenesis; establishment of mitotic spindle orientation; attachment of spindle microtubules to kinetochore during mitosis; mitotic sister chromatid segregation; cell division; mitotic cell cycle; attachment of spindle microtubules to kinetochore; chromosome segregation

size1 :

0.05 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage