Mouse Monoclonal [clone CF-145] (IgG2b) to Human S100A8 / MRP8 | MyBioSource MBS245035 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Mouse Monoclonal [clone CF-145] (IgG2b) to Human S100A8 / MRP8

catalog :

MBS245035

quantity :

0.05 mg

price :

495 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CF-145

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS245035

products type :

Antibody

products full name :

Mouse Monoclonal [clone CF-145] (IgG2b) to Human S100A8 / MRP8

products short name :

S100A8 / MRP8

products name syn :

Anti-S100A8 / MRP8 Antibody (clone CF-145) IHC-plus; S100A8; 60B8AG; CFAG; CAGA; Calgranulin A; Calgranulin-A; Calprotectin L1L subunit; Cystic fibrosis antigen; CGLA; L1Ag; NIF; p8; Protein S100-A8; MRP8; Urinary stone protein band A; CP-10; MA387; MRP-8; Human S100A8; MRP8

other names :

protein S100-A8; Protein S100-A8; protein S100-A8; MRP-8; calgranulin A; calgranulin-A; cystic fibrosis antigen; calprotectin L1L subunit; urinary stone protein band A; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium binding protein A8; Calgranulin-A; Calprotectin L1L subunit; Cystic fibrosis antigen; CFAG; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; S100 calcium-binding protein A8; Urinary stone protein band AProtein S100-A8, N-terminally processed

products gene name :

S100A8

products gene name syn :

MRP8

other gene names :

S100A8; S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; CAGA; CFAG; MRP8; CFAG; MRP-8; p8

uniprot entry name :

S10A8_HUMAN

clonality :

Monoclonal

isotype :

IgG2b

clone :

CF-145

host :

Mouse

reactivity :

Human

sequence length :

specificity :

Purified human Calgranulin A

purity :

Protein G Purified

form :

PBS, 0.02% sodium azide. Sourced in Ascites.

concentration :

1 mg/ml

storage stability :

Store at 4 degree C for short term applications. For long term storage, aliquot and store at -20 degree C.

tested application :

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)

app notes :

Flo, IF, IHC-P (2.5 ug/ml), WB. Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.

other info1 :

Target Species: Human. Immunogen Description: Purified granulocyte antigen of human origin

other info2 :

Immunogen Type: Purified protein. Immunogen: S100A8 / MRP8 antibody was raised against purified granulocyte antigen of human origin

products description :

S100 Calcium Binding Protein A8 (S100A8) is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A8 may function in the inhibition of casein kinase and as a cytokine.

ncbi gi num :

21614544

ncbi acc num :

NP_002955.2

ncbi gb acc num :

NM_002964.4

uniprot acc num :

P05109

ncbi mol weight :

10,835 Da

ncbi summary :

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq, Jul 2008]

uniprot summary :

S100A8: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Belongs to the S-100 family. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extracellular space; cytoskeleton; plasma membrane; extracellular region; cytosol; nucleus. Molecular Function: arachidonic acid binding; protein binding; RAGE receptor binding; zinc ion binding; microtubule binding; calcium ion binding. Biological Process: caspase activation; neutrophil chemotaxis; chemokine production; chronic inflammatory response; wound healing; cytokine production; positive regulation of peptide secretion; response to lipopolysaccharide; positive regulation of cell growth; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; sequestering of zinc ion; response to ethanol; response to zinc ion; defense response to bacterium; innate immune response; autophagy; inflammatory response; defense response to fungus; acute inflammatory response; regulation of cytoskeleton organization and biogenesis; positive regulation of inflammatory response

size1 :

0.05 mg

price1 :

495 USD

more info or order :

MyBioSource product webpage