antibody

Rabbit Polyclonal (IgG) to Human BAD

MBS244999

0.05 mg

495 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Rabbit Polyclonal (IgG) to Human BAD

BAD

Anti-BAD Antibody (aa119-168) IHC-plus; BAD; BBC2; Bcl-2-like protein 8; BCL2-binding component 6; BCL2L8; BBC6; BCL-X/BCL-2 binding protein; Bcl-2-binding component 6; Bcl2 antagonist of cell death; BCL2-binding protein; Bcl2-L-8; Human BAD

bcl2 antagonist of cell death; Bcl2-associated agonist of cell death; bcl2-associated agonist of cell death; bcl2-L-8; BCL2-binding protein; bcl-2-like protein 8; BCL2-binding component 6; bcl-2-binding component 6; BCL-X/BCL-2 binding protein; bcl2 antagonist of cell death; BCL2-antagonist of cell death protein; bcl-XL/Bcl-2-associated death promoter; BCL2-associated agonist of cell death; Bcl-2-binding component 6; Bcl-2-like protein 8; Bcl2-L-8; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death

BAD

BAD; BAD; BBC2; BCL2L8; BBC6; BCL2L8; BAD; Bcl2-L-8

BAD_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

168

BAD (Ab-155) Antibody detects endogenous levels of total BAD protein.

Immunoaffinity Purified

PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol

1 mg/ml

Store at -20 degree C for up to one year.

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

ELISA (1:10000), IHC-P (10 ug/ml), WB (1:500 - 1:1000). Optimal dilution to be determined by the researcher.

Target Species: Human. Immunogen Description: Synthetic peptide from human BAD (aa119-168). Target: BVL2-associdated agonist of cell death (BAD). Family/Subfamily: Apoptosis/ Not assigned-Other. Immunogen Species: BAD antibody was raised against Human. Presentation: PBS (without Mg2+, Ca2+), pH7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

Immunogen: BAD antibody was raised against synthetic peptide from human BAD (aa119-168). Antigen Modification: aa119-168

Family: Apoptosis

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax.

10835069

NP_004313.1

NM_004322.3

Q92934

18,392 Da

AKT Phosphorylates Targets In The Cytosol Pathway (106475); Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (366160); Alpha-synuclein Signaling Pathway (137913); Alpha6-Beta4 Integrin Signaling Pathway (198807); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509)

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]

BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL. Protein type: Apoptosis. Chromosomal Location of Human Ortholog: 11q13.1. Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol. Molecular Function: protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; lipid binding; protein kinase binding; protein phosphatase 2B binding. Biological Process: response to oleate; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of proteolysis; apoptosis; response to glucocorticoid stimulus; positive regulation of caspase activity; glucose homeostasis; cellular process regulating host cell cycle in response to virus; response to estradiol stimulus; positive regulation of apoptosis by virus; response to glucose stimulus; pore complex biogenesis; positive regulation of autophagy; positive regulation of glucokinase activity; caspase activation; epidermal growth factor receptor signaling pathway; response to drug; release of cytochrome c from mitochondria; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; positive regulation of insulin secretion; ADP metabolic process; response to testosterone stimulus; response to amino acid stimulus; ATP metabolic process; glucose catabolic process; response to ethanol; response to hydrogen peroxide; DNA damage response, signal transduction resulting in induction of apoptosis; induction of apoptosis via death domain receptors; positive regulation of T cell differentiation; positive regulation of B cell differentiation; innate immune response; regulation of mitochondrial membrane permeability; response to calcium ion; response to progesterone stimulus; positive regulation of epithelial cell proliferation

0.05 mg

495 USD