Rabbit Polyclonal (IgG) to Sheep YWHAE / 14-3-3 Epsilon | MyBioSource MBS244175 product information

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product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal (IgG) to Sheep YWHAE / 14-3-3 Epsilon

catalog :

MBS244175

quantity :

0.05 ml

price :

495 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken, cow

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

product information

catalog number :

MBS244175

products type :

Antibody

products full name :

Rabbit Polyclonal (IgG) to Sheep YWHAE / 14-3-3 Epsilon

products short name :

YWHAE / 14-3-3 Epsilon

products name syn :

Anti-YWHAE / 14-3-3 Epsilon Antibody (N-Terminus) IHC-plus; YWHAE; 14-3-3E; 14-3-3 epsilon; 14-3-3 protein epsilon; MDCR; MDS; KCIP-1; Sheep YWHAE; 14-3-3 Epsilon

other names :

14-3-3 protein epsilon; 14-3-3 protein epsilon; 14-3-3 protein epsilon; 14-3-3 epsilon; epididymis luminal protein 2; protein kinase C inhibitor protein-1; mitochondrial import stimulation factor L subunit; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon

products gene name :

YWHAE

other gene names :

YWHAE; YWHAE; MDS; HEL2; MDCR; KCIP-1; 14-3-3E; 14-3-3E

uniprot entry name :

1433E_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Bovine, Chicken, Human, Mouse, Rabbit, Rat, Sheep. Predicted Reactivity: Mammal (at least 90% immunogen sequence identity)

sequence length :

255

specificity :

Recognizes the acetylated N-terminal of 14-3-3 epsilon. It is a member of the 14-3-3 family which consists of 30 kD proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 epsilon is expressed in all mammals. It is deleted in small cell lung cancers disrupting the G(2) checkpoint. During apoptosis, cleavage of 14-3-3 protein by caspase-3 increases the affinity between bad and Bcl-x(L). When bound by ERK, 14-3-3 epsilon is thought to deactivate heat shock factor 1 in cells recovering from heat shock. It is also a potential suicide susceptibility gene. May not react with recombinant proteins that are not N-acetylated.

form :

Serum, 0.09% sodium azide.

storage stability :

+4 degree C or -20 degree C, Avoid repeated freezing and thawing.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

app notes :

ELISA, IHC-P (1:400), WB (1:3000). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.

other info1 :

Target Species: Sheep. Immunogen Description: Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 epsilon. (N-TERMINAL).

other info2 :

Immunogen Type: Synthetic peptide. Immunogen: YWHAE / 14-3-3 Epsilon antibody was raised against synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 epsilon. (N-TERMINAL). Antigen Modification: N-Terminus

ncbi gi num :

5803225

ncbi acc num :

NP_006752.1

ncbi gb acc num :

NM_006761.4

uniprot acc num :

P62258

ncbi mol weight :

26,504 Da

ncbi pathways :

Activation Of BAD And Translocation To Mitochondria Pathway 105659!!Activation Of BH3-only Proteins Pathway 105658!!Alpha6-Beta4 Integrin Signaling Pathway 198807!!Anchoring Of The Basal Body To The Plasma Membrane Pathway 1127503!!Apoptosis Pathway 105648!!Assembly Of The Primary Cilium Pathway 1127502!!Calcium Regulation In The Cardiac Cell Pathway 198906!!Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!Cell Cycle Pathway 198811

ncbi summary :

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]

size :

0.05 ml

price :

495 USD