Rabbit Polyclonal (IgG) to Human S1PR1 / EDG1 / S1P1 | MyBioSource MBS244124 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal (IgG) to Human S1PR1 / EDG1 / S1P1

catalog :

MBS244124

quantity :

0.05 mL

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-S1PR1 / EDG1 antibody IHC of human placenta, endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

product information

catalog number :

MBS244124

products type :

Antibody

products full name :

Rabbit Polyclonal (IgG) to Human S1PR1 / EDG1 / S1P1

products short name :

[S1PR1 / EDG1 / S1P1]

products name syn :

[Anti-S1PR1 / EDG1 / S1P1 Antibody IHC-plus; S1PR1; CD363; CD363 antigen; CHEDG1; ECGF1; EDG-1; D1S3362; EDG1; S1P1; S1P receptor Edg-1; S1P receptor 1; Human S1PR1; EDG1; S1P1]

other names :

[sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor 1; S1P receptor 1; S1P receptor Edg-1; sphingosine 1-phosphate receptor EDG1; sphingosine 1-phosphate receptor Edg-1; endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; sphingosine-1-phosphate receptor 1; Endothelial differentiation G-protein coupled receptor 1; Sphingosine 1-phosphate receptor Edg-1; S1P receptor Edg-1; CD_antigen: CD363]

products gene name :

[S1PR1]

products gene name syn :

[EDG1; S1P1]

other gene names :

[S1PR1; S1PR1; EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362; CHEDG1; EDG1; S1P receptor 1; S1P1; S1P receptor Edg-1]

uniprot entry name :

S1PR1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

382

specificity :

The S1P1 antibody is directed against a cytoplasmic loop of this receptor.

purity :

Affinity Purified

form :

PBS, pH 7.4, 0.02% sodium azide

storage stability :

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB)

app notes :

IHC-P (2.5 ug/ml). WB (1:2000). Optimal dilution to be determined by the researcher

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen Species: Antibody was raised against Human. Antigen Type: Synthetic peptide. Immunogen: Antibody was raised against synthetic peptide

other info2 :

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products categories :

Family: GPCR. Subfamily: Lysophospholipid/Lysosphingolipid

products description :

EDG1, a Lysophospholipid/Lysosphingolipid Receptor, binds sphingosine-1-phosphate and may control cell proliferation, survival, and suppression of apoptosis. Overexpression of EDG-1 leads to increased cell-cell aggregation and overexpression of cadherins.

ncbi gi num :

13027636

ncbi acc num :

NP_001391.2

ncbi gb acc num :

NM_001400.4

uniprot acc num :

P21453

ncbi mol weight :

42,811 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); Fc-epsilon Receptor I Signaling In Mast Cells Pathway (138050); FoxO Signaling Pathway (921162); G Alpha (i) Signalling Events Pathway (119550); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Other Pathway (198765); Lysosphingolipid And LPA Receptors Pathway (106376)

ncbi summary :

The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. [provided by RefSeq, Jul 2008]

uniprot summary :

EDG-1: a G protein-coupled receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Highly expressed in endothelial cells and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin. Suggested to be involved in the processes that regulate the migration/differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. S1P-induced endothelial cell migration requires AKT1-mediated phosphorylation of the third intracellular loop. Seems to be coupled to the G(i) subclass of heteromeric G proteins. Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR. Chromosomal Location of Human Ortholog: 1p21. Cellular Component: plasma membrane; integral to membrane; endosome; intrinsic to plasma membrane; lipid raft; external side of plasma membrane. Molecular Function: G-protein coupled receptor activity; sphingolipid binding; G-protein-coupled receptor binding. Biological Process: lamellipodium biogenesis; regulation of cell adhesion; endothelial cell differentiation; regulation of bone resorption; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); cell migration; negative regulation of stress fiber formation; positive regulation of positive chemotaxis; positive regulation of smooth muscle cell proliferation; transmission of nerve impulse; chemotaxis; blood vessel maturation; neuron differentiation; G-protein coupled receptor protein signaling pathway; actin cytoskeleton reorganization; positive regulation of transcription from RNA polymerase II promoter; G-protein signaling, adenylate cyclase inhibiting pathway; brain development; angiogenesis; cell adhesion; regulation of bone mineralization; positive regulation of cell migration

size1 :

0.05 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage