Rabbit Polyclonal (IgG) to Mouse TNFRSF1B / TNFR2 / p75 | MyBioSource MBS243925 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal (IgG) to Mouse TNFRSF1B / TNFR2 / p75

catalog :

MBS243925

quantity :

0.25 mL

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-TNFR2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

product information

catalog number :

MBS243925

products type :

Antibody

products full name :

Rabbit Polyclonal (IgG) to Mouse TNFRSF1B / TNFR2 / p75

products short name :

[TNFRSF1B / TNFR2 / p75]

products name syn :

[TNFRSF1B Antibody, CD120b antigen Antibody, CD120b Antibody, Etanercept Antibody, p75 TNF receptor Antibody, Soluble TNFR1B variant 1 Antibody, TNFBR Antibody, Tumor necrosis factor receptor Antibody, TNF-R-II Antibody, TNF-R75 Antibody, TNFR2 Antibody, p75TNFR Antibody, p80 TNF-alpha receptor Antibody, TBPII Antibody, TNF-R2 Antibody, TNFR80 Antibody, TNF-RII Antibody, TNFR-II Antibody, TNFR1B Antibody]

other names :

[tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily member 1B; TNF-R2; TNF-RII; p75 TNF receptor; p80 TNF-alpha receptor; soluble TNFR1B variant 1; tumor necrosis factor receptor 2; tumor necrosis factor beta receptor; tumor necrosis factor receptor type II; tumor necrosis factor binding protein 2; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha receptor; CD_antigen: CD120b; INN: EtanerceptCleaved into the following 2 chains:Tumor necrosis factor receptor superfamily member 1b, membrane form; Tumor necrosis factor-binding protein 2Alternative name(s):TBP-2; TBPII]

products gene name :

[TNFRSF1B]

products gene name syn :

[TNFR2; p75]

other gene names :

[TNFRSF1B; TNFRSF1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II; TNFBR; TNFR2; TNF-R2; TNF-RII; TNFR-II]

uniprot entry name :

TNR1B_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse, Rat

sequence length :

461

purity :

Immunoaffinity Purified

form :

10 mM PBS, pH 7.4, BSA, with less than 0.1% sodium azide.

storage stability :

Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

tested application :

Immunohistochemistry, Immunohistochemistry (IHC - Paraffin)

app notes :

IHC-P (1:50). (Optimal dilution to be determined by the researcher).

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Target Species: Mouse. Immunogen Description: A synthetic peptide derived from C-terminal of mouse TNF-R2. Family/Subfamily: TNF Receptor/not assigned-TNF Receptor.

other info2 :

Immunogen Type: Synthetic peptide. Immunogen: TNFRSF1B / TNFR2 antibody was raised against a synthetic peptide derived from C-terminal of mouse TNF-R2. Epitope: C-Terminus. Target: Mouse TNFRSF1B/TNFR2. Immunogen Species: TNFRSF1B/TNFR2 antibody was raised against Mouse. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products categories :

Family: TNF Receptor

products description :

TNFR2 is a 75 kD transmembrane glycoprotein that also exists as a secreted protein. TNF receptors self assemble through a distinct functional extracellular domain termed PLAD in the absence of ligand. Efficient TNFA binding depends on receptor self assembly. TNFR2 has been shown to be responsible for stimulation of activated T lymphocytes by TNFalpha.

ncbi gi num :

4507577

ncbi acc num :

NP_001057.1

ncbi gb acc num :

NM_001066.2

uniprot acc num :

P20333

ncbi pathways :

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (198797); Apoptosis Modulation And Signaling Pathway (198822); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL-3 Signaling Pathway (198881); Inflammatory Response Pathway (198766)

ncbi summary :

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008]

uniprot summary :

TNF-R2: Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. Binds to TRAF2. Interacts with BMX. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, cytokine. Chromosomal Location of Human Ortholog: 1p36.22. Cellular Component: cell soma; perinuclear region of cytoplasm; integral to membrane; extracellular region; plasma membrane; nucleus; lipid raft. Molecular Function: protein binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding. Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; tumor necrosis factor-mediated signaling pathway; negative regulation of inflammatory response; immune response; inflammatory response; RNA destabilization; positive regulation of membrane protein ectodomain proteolysis; aging

size1 :

0.25 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage