Rabbit Polyclonal (IgG) to Human RREB1 | MyBioSource MBS243911 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal (IgG) to Human RREB1

catalog :

MBS243911

quantity :

0.05 mL

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-RREB1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

image 2 :

Anti-RREB1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

product information

catalog number :

MBS243911

products type :

Antibody

products full name :

Rabbit Polyclonal (IgG) to Human RREB1

products short name :

[RREB1]

products name syn :

[Anti-RREB1 Antibody (aa560-609) IHC-plus; RREB1; DNA-binding protein; FINB; Hindsight homolog; Zep-1; HNT; LZ321; RREB-1; Human RREB1]

other names :

[ras-responsive element-binding protein 1 isoform 1; Ras-responsive element-binding protein 1; ras-responsive element-binding protein 1; hindsight homolog; DNA-binding protein; finger protein in nuclear bodies; raf-responsive zinc finger protein LZ321; zinc finger motif enhancer binding protein 1; zinc finger motif enhancer-binding protein 1; zinc finger motif-enhancer binding-protein 1; ras responsive element binding protein 1; Finger protein in nuclear bodies; Raf-responsive zinc finger protein LZ321; Zinc finger motif enhancer-binding protein 1; Zep-1]

products gene name :

[RREB1]

other gene names :

[RREB1; RREB1; HNT; FINB; LZ321; Zep-1; RREB-1; FINB; RREB-1; Zep-1]

uniprot entry name :

RREB1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

1742

specificity :

RREB1 Antibody detects endogenous levels of total RREB1 protein.

purity :

Immunoaffinity Purified

form :

PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol

concentration :

1 mg/ml

storage stability :

Store at -20 degree C for up to one year.

tested application :

Immunohistochemistry (IHC - Paraffin), ELISA (EIA)

app notes :

ELISA (1:20000). IHC-P (1:100). Optimal dilution to be determined by the researcher.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Target Species: Human. Immunogen Description: Synthetic peptide from human RREB1 (aa560-609).

other info2 :

Immunogen Species: RREB1 antibody was raised against Human. Antigen Type: Synthetic peptide. Immunogen: RREB1 antibody was raised against synthetic peptide from human RREB1 (aa560-609). Antigen Modification: aa560-609. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products description :

RREB1is a zinc finger transcription factor that binds to RAS-responsive elements (RREs) of gene promoters. It has been shown that the calcitonin gene promoter contains an RRE and that the encoded protein binds there and increases expression of calcitonin, which may be involved in Ras/Raf-mediated cell differentiation. Multiple transcript variants encoding several different isoforms have been found for this gene.

ncbi gi num :

51173737

ncbi acc num :

NP_001003699.1

ncbi gb acc num :

NM_001003699.3

uniprot acc num :

Q92766

ncbi mol weight :

34,614 Da

ncbi summary :

The protein encoded by this gene is a zinc finger transcription factor that binds to RAS-responsive elements (RREs) of gene promoters. It has been shown that the calcitonin gene promoter contains an RRE and that the encoded protein binds there and increases expression of calcitonin, which may be involved in Ras/Raf-mediated cell differentiation. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Dec 2009]

uniprot summary :

RREB1: Transcription factor that binds specifically to the RAS- responsive elements (RRE) of gene promoters. May be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. Represses the angiotensinogen gene. Negatively regulates the transcriptional activity of AR. Potentiates the transcriptional activity of NEUROD1. Belongs to the krueppel C2H2-type zinc-finger protein family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Motility/polarity/chemotaxis; DNA-binding; C2H2-type zinc finger protein. Chromosomal Location of Human Ortholog: 6p25. Cellular Component: nucleoplasm; nuclear body; cytoplasm; nucleolus; nuclear speck; nucleus. Molecular Function: metal ion binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription, DNA-dependent; Ras protein signal transduction; positive regulation of mammary gland epithelial cell proliferation; negative regulation of transcription from RNA polymerase II promoter

size1 :

0.05 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage