antibody

Rabbit Polyclonal to Human GPR65 / TDAG8

MBS243866

0.05 mg

530 USD

polyclonal

rabbit

nonconjugated

immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Rabbit Polyclonal to Human GPR65 / TDAG8

[GPR65 / TDAG8]

[GPR65 Antibody, G-protein coupled receptor 65 Antibody, Gpcr25 Antibody, HTDAG8 Antibody, TDAG8 Antibody, T-cell death-associated gene 8 Antibody, Psychosine receptor Antibody, G protein-coupled receptor 65 Antibody]

[psychosine receptor; Psychosine receptor; psychosine receptor; G-protein coupled receptor 65; T-cell death-associated gene 8 protein; G protein-coupled receptor 65; G-protein coupled receptor 65; T-cell death-associated gene 8 protein]

[GPR65]

[TDAG8]

[GPR65; GPR65; TDAG8; hTDAG8; TDAG8]

PSYR_HUMAN

Polyclonal

Rabbit

Human. Predicted Reactivity: Gorilla, Gibbon (at least 90% immunogen sequence identity)

337

Human GPR65. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except VCL (50%), CPS1 (39%).

Immunoaffinity Purified

PBS, less than 0.1% sodium azide.

1 mg/ml

Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.

Immunohistochemistry (IHC - Paraffin)

IHC-P (10 ug/ml). Optimal dilution to be determined by the researcher.

Immunohistochemistry (IHC)

Immunogen Species: Human. Immunogen Description: Synthetic 18 amino acid peptide from 3rd extracellular domain of human GPR65. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Gibbon (94%).

Immunogen Type: Synthetic peptide. Immunogen: GPR65 / TDAG8 antibody was raised against synthetic 18 amino acid peptide from 3rd extracellular domain of human GPR65. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Gibbon (94%). Antigen Modification: Extracellular Domain. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

Family: GPCR. Subfamily: Lysophospholipid/Lysosphingolipid

GPR65 (TDAG8), a Lysophospholipid/Lysosphingolipid Receptor, is a molecular target for psychosine, a toxic lipid formed by the breakdown of galactosylceramide (cerebroside). A build-up of psychosine, caused by the lack of galactosylceramidase, results in the death of the myelin-synthesizing cells, oligodendrocytes, which is associated with Krabbe disease.

33695104

NP_003599.2

NM_003608.3

Q8IYL9

39,333 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637); Signal Transduction Pathway (477114)

GPR65: Receptor for the glycosphingolipid psychosine (PSY) and several related glycosphingolipids. May have a role in activation- induced cell death or differentiation of T-cells. Belongs to the G-protein coupled receptor 1 family. Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1. Chromosomal Location of Human Ortholog: 14q31-q32.1. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; apoptosis; multicellular organismal development; positive regulation of stress fiber formation; actin cytoskeleton reorganization; positive regulation of cAMP biosynthetic process; immune response; response to acidity

0.05 mg

530 USD