antibody

Rabbit Polyclonal (IgG) to Human PTP1B

MBS243622

0.05 mg

495 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Rabbit Polyclonal (IgG) to Human PTP1B

PTP1B

Anti-PTP1B Antibody (aa16-65) IHC-plus; PTPN1; PTP1B; PTP-1B; PTPase 1B; Human PTP1B

tyrosine-protein phosphatase non-receptor type 1 isoform 1; Tyrosine-protein phosphatase non-receptor type 1; tyrosine-protein phosphatase non-receptor type 1; protein-tyrosine phosphatase 1B; protein tyrosine phosphatase, placental; protein tyrosine phosphatase, non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP-1B

PTP1B

PTPN1

PTPN1; PTPN1; PTP1B; PTP1B; PTP-1B

PTN1_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

435

PTP1B Antibody detects endogenous levels of total PTP1B protein.

Immunoaffinity Purified

PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol

1 mg/ml

Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

ELISA (1:20000), IHC-P (10 ug/ml), WB (1:500 - 1:1000)

Target: PTP1B. Target Species: Human. Immunogen Description: Synthetic peptide from human PTP1B (aa16-65). Immunogen Species: PTP1B antibody was raised against Human

Immunogen: PTP1B antibody was raised against synthetic peptide from human PTP1B (aa16-65). Antigen Modification: aa16-65

Family: Protein Phosphatase. Subfamily: NT1

PTP1B, a NT1 non-receptor protein tyrosine phosphatase, has been shown to regulate insulin signaling, integrin signaling, and leptin signaling. It has been shown that JAK2 and TYK2 are physiological substrates of PTP1B and that the substrate recognition site within theses kinases is similar to the site of dephosphorylation identified within the insulin receptor.The knowledge that mice lacking PTP1B are hypersensitive to insulin and resistant to obesity and that insulin signaling can be enhanced by the inhibition of PTP1B have made PTP1B a potential therapy target against type 2 diabetes and obesity.

4506289

NP_002818.1

NM_002827.3

P18031

49,967 Da

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Cytokine Signaling In Immune System Pathway (366171); EGF Receptor (ErbB1) Signaling Pathway (137970); Growth Hormone Receptor Signaling Pathway (477128); Hemostasis Pathway (106028); IGF1 Pathway (137992); Immune System Pathway (106386); Insulin Pathway (138026)

The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

PTP1B: a non-receptor phospho-tyrosine protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation and inactivation of PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. Negatively regulates insulin signaling by dephosphorylating the phosphotyrosine residues of insulin receptor. Also reported to dephosphorylate integrin, epidermal growth factor receptor, JAK2 and TYK2, regulating cell growth control and the cellular response to interferon. May regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes. Protein type: Motility/polarity/chemotaxis; Phosphatase; EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor). Chromosomal Location of Human Ortholog: 20q13.1-q13.2. Cellular Component: endoplasmic reticulum; early endosome; plasma membrane; cytoplasmic vesicle; cytosol. Molecular Function: protein binding; enzyme binding; ephrin receptor binding; zinc ion binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; protein kinase binding; insulin receptor binding. Biological Process: platelet activation; unfolded protein response, activation of signaling protein activity; negative regulation of vascular endothelial growth factor receptor signaling pathway; unfolded protein response; cytokine and chemokine mediated signaling pathway; actin cytoskeleton reorganization; regulation of endocytosis; insulin receptor signaling pathway; negative regulation of insulin receptor signaling pathway; regulation of signal transduction; blood coagulation. Disease: Diabetes Mellitus, Noninsulin-dependent

0.05 mg

495 USD