Goat Polyclonal to Human UBE2O | MyBioSource MBS243213 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat Polyclonal to Human UBE2O

catalog :

MBS243213

quantity :

0.05 mg

price :

530 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, rat

application :

ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-UBE2O antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

product information

catalog number :

MBS243213

products type :

Antibody

products full name :

Goat Polyclonal to Human UBE2O

products short name :

[UBE2O]

products name syn :

[UBE2O Antibody, KIAA1734 Antibody, Ubiquitin-protein ligase O Antibody, Ubiquitin carrier protein O Antibody, E2-230K Antibod]

other names :

[ubiquitin-conjugating enzyme E2 O; E2/E3 hybrid ubiquitin-protein ligase UBE2O; E2/E3 hybrid ubiquitin-protein ligase UBE2O; ubiquitin-protein ligase O; ubiquitin carrier protein O; ubiquitin-conjugating enzyme E2-230K; ubiquitin-conjugating enzyme E2 of 230 kDa; ubiquitin-conjugating enzyme E2O; Ubiquitin carrier protein O; Ubiquitin-conjugating enzyme E2 O; Ubiquitin-conjugating enzyme E2 of 230 kDa; Ubiquitin-conjugating enzyme E2-230K; Ubiquitin-protein ligase O]

products gene name :

[UBE2O]

other gene names :

[UBE2O; UBE2O; E2-230K; KIAA1734; Ubiquitin-conjugating enzyme E2-230K]

uniprot entry name :

UBE2O_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Human, Monkey (tested or 100% immunogen sequence identity). Predicted Reactivity: Mouse, Rat, Hamster (at least 90% immunogen sequence identity)

sequence positions :

[aa1279-1292]

sequence length :

1292

specificity :

Human UBE2O.

purity :

Immunoaffinity Purified

form :

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

concentration :

0.5 mg/ml

storage stability :

Store at -20 degree C. Minimize freezing and thawing.

tested application :

Immunohistochemistry (IHC - Paraffin), ELISA (EIA)

app notes :

ELISA (1:32000). IHC-P (3.75 ug/ml). (Optimal dilution to be determined by the researcher.)

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Target: Human UBE2O. Immunogen Description: Synthetic peptide ALLEAGMPECTEDK from the C-terminus of human UBE2O (NP_071349.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Mouse, Rat, Hamster, Panda (93%); Dog, Horse (86%).

other info2 :

Immunogen Type: Synthetic peptide. Immunogen: UBE2O antibody was raised against synthetic peptide ALLEAGMPECTEDK from the C-terminus of human UBE2O (NP_071349.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Mouse, Rat, Hamster, Panda (93%); Dog, Horse (86%). Antigen Modification: C-Terminus. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

ncbi gi num :

192449449

ncbi acc num :

NP_071349.3

ncbi gb acc num :

NM_022066.3

uniprot acc num :

Q9C0C9

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Immune System Pathway (106386); Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)

uniprot summary :

UBE2O: Catalyzes the covalent attachment of ubiquitin to other proteins. Belongs to the ubiquitin-conjugating enzyme family. Protein type: EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Ligase. Chromosomal Location of Human Ortholog: 17q25.1. Cellular Component: nucleoplasm; cytoplasm; nucleus. Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity. Biological Process: protein monoubiquitination; retrograde transport, endosome to Golgi; positive regulation of BMP signaling pathway

size1 :

0.05 mg

price1 :

530 USD

more info or order :

MyBioSource product webpage