Rabbit Polyclonal to Human TACR3 / NK3R | MyBioSource MBS243070 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal to Human TACR3 / NK3R

catalog :

MBS243070

quantity :

0.05 mg

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-TACR3 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

product information

catalog number :

MBS243070

products type :

Antibody

products full name :

Rabbit Polyclonal to Human TACR3 / NK3R

products short name :

[TACR3 / NK3R]

products name syn :

[Anti-TACR3 / NK3R Antibody (N-Terminus) IHC-plus; TACR3; HH11; Nmk-r; NK3R; NKR; Tachykinin receptor 3; Neurokinin B; Neurokinin B receptor; NK-3 receptor; NK-3R; TAC3R; Neurokinin 3 receptor; Neurokinin beta receptor; Neuromedin-K receptor; TAC3RL; Human TACR3; NK3R]

other names :

[neuromedin-K receptor; Neuromedin-K receptor; neuromedin-K receptor; NK-3 receptor; neurokinin B receptor; neurokinin beta receptor; tachykinin receptor 3; NK-3 receptor; NK-3R; Neurokinin B receptor; Tachykinin receptor 3]

products gene name :

[TACR3]

products gene name syn :

[NK3R]

other gene names :

[TACR3; TACR3; NKR; HH11; NK3R; NK-3R; TAC3RL; NK3R; TAC3R; NKR; NK-3R]

uniprot entry name :

NK3R_HUMAN

clonality :

polyclonal

host :

rabbit

reactivity :

Human. Predicted: Monkey

sequence length :

465

specificity :

Human TACR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

purity :

Immunoaffinity Purified

form :

PBS, 0.1% sodium azide.

concentration :

1 mg/ml

storage stability :

Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze/thaw cycles.

tested application :

Immunohistochemistry, Immunohistochemistry (IHC - Paraffin)

app notes :

Immunohistochemistry-Paraffin (IHC-P) (8 ug/ml). Optimal dilution to be determined by the researcher.

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Target Species: TACR3/NK3R . Immunogen Species: TACR3/NK3R antibody was raised against Human. Antigen Type: Synthetic peptide. Immunogen: TACR3 / NK3R antibody was raised against synthetic 20 amino acid peptide from N-terminal extracellular domain of human TACR3. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (95%). Epitope: N-Terminus

other info2 :

. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip. Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.

products categories :

Family: GPCR. Subfamily: Tachykinin

products description :

NK3R is a Tachykinin Receptor specific for neurokinin B, although its function is not well understood.

ncbi gi num :

7669548

ncbi acc num :

NP_001050.1

ncbi gb acc num :

NM_001059.2

uniprot acc num :

P29371

ncbi pathways :

Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637)

ncbi summary :

This gene belongs to a family of genes that function as receptors for tachykinins. Receptor affinities are specified by variations in the 5'-end of the sequence. The receptors belonging to this family are characterized by interactions with G proteins and 7 hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin neurokinin 3, also referred to as neurokinin B. [provided by RefSeq, Jul 2008]

uniprot summary :

TACR3: This is a receptor for the tachykinin neuropeptide neuromedin-K (neurokinin B). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: neuromedin-K substance K substance P. Belongs to the G-protein coupled receptor 1 family. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1. Chromosomal Location of Human Ortholog: 4q25. Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; nucleus. Molecular Function: protein binding; tachykinin receptor activity. Biological Process: tachykinin signaling pathway; hyperosmotic salinity response; regulation of dopamine metabolic process; response to morphine; positive regulation of heart rate; response to cocaine; positive regulation of blood pressure; response to estradiol stimulus; aging. Disease: Hypogonadotropic Hypogonadism 11 With Or Without Anosmia

size1 :

0.05 mg

price1 :

530 USD

more info or order :

MyBioSource product webpage