antibody

Goat Polyclonal to Human UCP2

MBS242664

0.05 mg

530 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Goat Polyclonal to Human UCP2

[UCP2]

[Anti-UCP2 Antibody (Internal) IHC-plus; UCP2; BMIQ4; UCP 2; Uncoupling protein 2; UCPH; SLC25A8; Human UCP2]

[mitochondrial uncoupling protein 2; Mitochondrial uncoupling protein 2; mitochondrial uncoupling protein 2; solute carrier family 25 member 8; uncoupling protein 2 (mitochondrial, proton carrier); Solute carrier family 25 member 8; UCPH]

[UCP2]

[UCP2; UCP2; UCPH; BMIQ4; SLC25A8; SLC25A8; UCP 2]

UCP2_HUMAN

polyclonal

goat

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Xenopus. Predicted Reactivity: Zebrafish

309

Human UCP2.

Immunoaffinity Purified

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

0.5 mg/mL

Store at -20°C. Avoid freezing and thawing.

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

IHC- Paraffin(3.75 ug/ml). Western blot (1-3ug/mL). ELISA (1:32000). Optimal dilution to be determined by the researcher).

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Western Blot (WB)

Antibody (0.5 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

. Immunogen Species: UCP2 antibody was raised against Human. Antigen Type: Synthetic peptide. Immunogen: UCP2 antibody was raised against synthetic peptide C-DSVKQFYTKGSEH from an internal region of human UCP2 (NP_003346.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Xenopus, Stickleback (100%); Smelt, Zebrafish (92%); Trout, Salmon (85%). Antigen Modification: Internal. Epitope: aa101-113. Usage summary: Immunohistochemistry: MBS242664 was validated for use in imunohistochemistry on a panel of 21 formalin-fixed, paraffin- embedded (FFPE)human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody,slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specifity. The optimal working concentration for MBS242664 was determined to be 3.75 ug/mL.

. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

Family: Transporter. Subfamily: Mitochondrial carrier

13259541

NP_003346.2

NM_003355.2

P55851

Electron Transport Chain Pathway (198860); Energy Metabolism Pathway (198907); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Metabolism Pathway (477135); Mitochondrial Uncoupling Proteins Pathway (160963); Respiratory Electron Transport, ATP Synthesis By Chemiosmotic Coupling, And Heat Production By Uncoupling Proteins. Pathway (105921); The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway (477137); The Fatty Acid Cycling Model Pathway (160964); The Proton Buffering Model Pathway (160965)

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'. [provided by RefSeq, Jul 2008]

UCP2: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. Belongs to the mitochondrial carrier family. Protein type: Transporter; Membrane protein, integral; Mitochondrial; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: mitochondrial inner membrane; integral to membrane. Biological Process: cellular metabolic process; proton transport; response to superoxide; cellular response to insulin stimulus; regulation of mitochondrial membrane potential; cellular response to amino acid starvation; transport; response to hypoxia; female pregnancy; mitochondrial transport; negative regulation of apoptosis; aging. Disease: Body Mass Index Quantitative Trait Locus 4

0.05 mg

530 USD