antibody

Rabbit Polyclonal (IgG) to Human APOA2 / Apolipoprotein A II

MBS242643

0.05 mg

530 USD

polyclonal

rabbit

nonconjugated

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Rabbit Polyclonal (IgG) to Human APOA2 / Apolipoprotein A II

[APOA2 / Apolipoprotein A II]

[Anti-APOA2 / Apolipoprotein A II Antibody IHC-plus; APOA2; Apo-AII; ApoAII; ApoA-II; Apolipoprotein A-II; Apolipoprotein A2; Apolipoprotein A II; Human APOA2; Apolipoprotein A II]

[apolipoprotein A-II preproprotein; Apolipoprotein A-II; apolipoprotein A-II; apolipoprotein A2; apolipoprotein A-II; Apolipoprotein A2Cleaved into the following 2 chains:Proapolipoprotein A-II; ProapoA-II; Truncated apolipoprotein A-IIAlternative name(s):Apolipoprotein A-II(1-76)]

[APOA2]

[APOA2; APOA2; apoAII; Apo-AII; ApoA-II; Apo-AII; ApoA-II; ProapoA-II]

APOA2_HUMAN

polyclonal

IgG

rabbit

Human

100

Specifically binds to human Apo AII.

Affinity Purified

PBS,pH 7.2, 0.05mM EDTA, 0.02% sodium azide.

1 mg/ml

Short term: 4°C. Long term: -20°C . Avoid freeze/thaw cycles.

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

Immunohistochemistry-Paraffin (5 ug/mL). Western Blot 1:5000-1:40000 . ELISA (EIA) 1:5000-1:40000. Optimal dilution to be determined by the researcher. Usage summary: Immunohistochemistry: MBS242643 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat indued antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specifity. The optimal concentration for MBS242643 was determined to be 5 ug/ml.

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Target Species: Apolipoprotein A-II. Immunogen Species: Apolipoprotein A-II antibody was raised against Human. Immunogen: APOA2 / Apolipoprotein A II antibody was raised against human Apo AII.

Disclaimer: Due to the highy specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip. Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.

Apolipoprotein (Apo-) A-II is the second most abundant protein of the high density lipoprotein particles. The apo-A-II gene consists of 4 exons and 3 introns. The four exons encode the 5' untranslated region, pre-peptide, a short N-terminal domain and a C-terminal domain composed of a variable number of lipid-binding amphipathic helices.

4502149

NP_001634.1

NM_001643.1

P02652

Chylomicron-mediated Lipid Transport Pathway (106157); Complement And Coagulation Cascades Pathway (198880); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); PPAR Signaling Pathway (83042)

This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. [provided by RefSeq, Jul 2008]

APOA2: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. Belongs to the apolipoprotein A2 family. Protein type: Endoplasmic reticulum; Secreted, signal peptide; Secreted; Lipid-binding. Chromosomal Location of Human Ortholog: 1q23.3. Cellular Component: chylomicron; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol. Molecular Function: lipid transporter activity; protein binding; protein homodimerization activity; lipase inhibitor activity; protein heterodimerization activity; cholesterol transporter activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; high-density lipoprotein binding; lipid binding; apolipoprotein receptor binding. Biological Process: positive regulation of catalytic activity; phototransduction, visible light; viral reproduction; protein folding; negative regulation of cholesterol transport; response to glucocorticoid stimulus; regulation of protein stability; diacylglycerol catabolic process; positive regulation of lipid catabolic process; cellular lipid metabolic process; negative regulation of lipase activity; regulation of cholesterol absorption; phospholipid efflux; negative regulation of lipid catabolic process; response to glucose stimulus; retinoid metabolic process; response to drug; cholesterol metabolic process; organ regeneration; protein amino acid oxidation; phospholipid catabolic process; positive regulation of interleukin-8 biosynthetic process; cholesterol efflux; lipoprotein metabolic process; negative regulation of cytokine secretion during immune response; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; response to estrogen stimulus; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; acute inflammatory response. Disease: Hypercholesterolemia, Familial

0.05 mg

530 USD