antibody

Goat Polyclonal to Mouse APOA1 / Apolipoprotein A 1

MBS242412

0.05 mg

495 USD

polyclonal

goat

nonconjugated

human, mouse

western blot, ELISA, immunohistochemistry, immunoprecipitation, enzyme immunoassay

Antibody

Goat Polyclonal to Mouse APOA1 / Apolipoprotein A 1

APOA1 / Apolipoprotein A 1

Anti-APOA1 / Apolipoprotein A 1 Antibody IHC-plus; APOA1; ApoA-I; APOAI; Apolipoprotein A-I; Apolipoprotein A1; Apo-AI; Mouse APOA1; Apolipoprotein A 1

apolipoprotein A-I preproprotein; Apolipoprotein A-I; apolipoprotein A-I; apo-AI; apolipoprotein A-I; Apolipoprotein A1Cleaved into the following 2 chains:Proapolipoprotein A-I; ProapoA-I; Truncated apolipoprotein A-IAlternative name(s):Apolipoprotein A-I(1-242)

APOA1

APOA1; APOA1; Apo-AI; ApoA-I; ProapoA-I

APOA1_HUMAN

Polyclonal

Goat

Mouse

267

Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross- reaction of anti-apoLipoprotein antibodies with other mouse serum proteins is negligible

Affinity Purified

0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, 0.01% sodium azide. For extended storage, mix, an equal volume of glycerol.

1 mg/ml

+4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)

ELISA (1:5000 - 1:10000), IHC-P (5 ug/ml), IP, WB (1:500 - 1:1000). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel formalin-fixed, paraffin-embedded (FFPE) mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody,.

Target Species: Mouse. Immunogen Description: Apolipoprotein Type A-I was isolated from mouse plasma by density gradient centrifugation followed by HPLC purification.

Immunogen: APOA1 / Apolipoprotein A 1 antibody was raised against apolipoprotein Type A-I was isolated from mouse plasma by density gradient centrifugation followed by HPLC purification.

APOA1 promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18-amino acid signal peptide is removed co-translationally and a 6-amino acid propeptide is cleaved post-translationally.

4557321

NP_000030.1

NM_000039.1

P02647

30,778 Da

ABC-family Proteins Mediated Transport Pathway (106573); ABCA Transporters In Lipid Homeostasis Pathway (477112); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Amyloids Pathway (366238); Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911)

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq, Jul 2008]

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family. Protein type: Cell development/differentiation; Lipid-binding; Secreted, signal peptide; Secreted; Endoplasmic reticulum; Vesicle; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 11q23-q24. Cellular Component: extracellular space; chylomicron; cell surface; endocytic vesicle; endoplasmic reticulum lumen; early endosome; plasma membrane; extracellular region; cytoplasmic vesicle; cytosol; nucleus; vesicle. Molecular Function: identical protein binding; lipase inhibitor activity; beta-amyloid binding; cholesterol binding; phosphatidylcholine binding; high-density lipoprotein binding; protein binding; enzyme binding; phospholipid transporter activity; cholesterol transporter activity; chemorepellent activity; phospholipid binding; apolipoprotein A-I receptor binding; apolipoprotein receptor binding. Biological Process: phototransduction, visible light; negative chemotaxis; negative regulation of lipase activity; axon regeneration in the peripheral nervous system; negative regulation of interleukin-1 beta secretion; sequestering of lipid; regulation of cholesterol absorption; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; response to drug; cholesterol metabolic process; platelet activation; organ regeneration; regulation of Cdc42 protein signal transduction; adrenal gland development; positive regulation of hydrolase activity; positive regulation of Rho protein signal transduction; lipoprotein metabolic process; positive regulation of transferase activity; vitamin transport; cholesterol biosynthetic process; negative regulation of cytokine secretion during immune response; cholesterol homeostasis; lipoprotein biosynthetic process; response to estrogen stimulus; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; positive regulation of lipoprotein lipase activity; blood vessel endothelial cell migration; cellular lipid metabolic process; platelet degranulation; phospholipid efflux; retinoid metabolic process; transmembrane transport; response to nutrient; phospholipid homeostasis; integrin-mediated signaling pathway; positive regulation of fatty acid biosynthetic process; receptor-mediated endocytosis; regulation of protein amino acid phosphorylation; cholesterol transport; negative regulation of heterotypic cell-cell adhesion; protein amino acid oxidation; protein stabilization; neurite regeneration; cholesterol efflux; glucocorticoid metabolic process; G-protein coupled receptor protein signaling pathway; reverse cholesterol transport; negative regulation of inflammatory response; endothelial cell proliferation; blood coagulation. Disease: Hypoalphalipoproteinemia, Primary; Amyloidosis, Familial Visceral

0.05 mg

495 USD