antibody

Goat Polyclonal to Human KMT2B / MLL4

MBS242382

0.05 mg

530 USD

polyclonal

goat

nonconjugated

human, mouse, rat, cow

immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Goat Polyclonal to Human KMT2B / MLL4

[KMT2B / MLL4]

[Anti-KMT2B / MLL4 Antibody (C-Terminus) IHC-plus; KMT2B; Lysine N-methyltransferase 2D; MLL4; HRX2; KMT2D; WBP-7; WW domain binding protein 7; Trithorax homologue 2; WBP7; KIAA0304; Lysine N-methyltransferase 2B; Trithorax homolog 2; TRX2; WW domain-binding protein 7; Human KMT2B; MLL4]

[histone-lysine N-methyltransferase 2B; Histone-lysine N-methyltransferase 2B; histone-lysine N-methyltransferase 2B; trithorax homologue 2; WW domain binding protein 7; mixed lineage leukemia gene homolog 2; histone-lysine N-methyltransferase MLL4; myeloid/lymphoid or mixed-lineage leukemia protein 4; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila) 4; lysine (K)-specific methyltransferase 2B; Myeloid/lymphoid or mixed-lineage leukemia protein 4; Trithorax homolog 2; WW domain-binding protein 7; WBP-7]

[KMT2B]

[MLL4]

[KMT2B; KMT2B; HRX2; MLL2; MLL4; TRX2; WBP7; MLL1B; WBP-7; HRX2; KIAA0304; MLL2; MLL4; TRX2; WBP7; Lysine N-methyltransferase 2B; WBP-7]

KMT2B_HUMAN

Polyclonal

Goat

Human, Monkey, Mouse, Rat, Bovine, Pig, Zebrafish. Predicted: Xenopus

2715

Human MLL4.

Immunoaffinity Purified

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

0.5 mg/ml

Store at -20°C. Minimize freezing and thawing.

Immunohistochemistry (IHC), Immunohistochemistry-Paraffin

IHC-Paraffin (5 ug/mL). Peptide Enzyme-Linked Immunosorbent Assay (1:128000). Optimal dilution to be determined by the researcher).

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunogen Description: Synthetic peptide CNCGAKRCRRFLN from the C-terminus of human MLL4 (NP_055542.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Pig, Zebrafish (100%); Xenopus, Pufferfish (92%). Epitope: aa2703-2715. Antigen Type: Synthetic peptide. Immunogen: KMT2B / MLL4 antibody was raised against synthetic peptide CNCGAKRCRRFLN from the C-terminus of human MLL4 (NP_055542.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Pig, Zebrafish (100%); Xenopus, Pufferfish (92%); Chimpanzee, Elephant, Panda, Dog, Bat, Horse, Opossum, Turkey, Chicken (85%). Usage Summary: Immunohistochemistry: MBS242382 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm stainig specifity. The optimal working concentration for MBS242382 was determined to be 5 ug/mL.

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip . Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation

This gene encodes a protein which contains multiple domains including a CXXC zinc finger, three PHD zinc fingers, two FY-rich domains, and a SET (suppressor of variegation, enhancer of zeste, and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This gene is ubiquitously expressed in adult tissues. It is also amplified in solid tumor cell lines, and may be involved in human cancer.

7662046

NP_055542.1

NM_014727.2

Q9UMN6

Chromatin Modifying Enzymes Pathway (1127682); Chromatin Organization Pathway (1127681); Lysine Degradation Pathway (82956); Lysine Degradation Pathway (320); PKMTs Methylate Histone Lysines Pathway (1127686)

This gene encodes a protein which contains multiple domains including a CXXC zinc finger, three PHD zinc fingers, two FY-rich domains, and a SET (suppressor of variegation, enhancer of zeste, and trithorax) domain. The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. This gene is ubiquitously expressed in adult tissues. It is also amplified in solid tumor cell lines, and may be involved in human cancer. Two alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene, however, the full length nature of the shorter transcript is not known. [provided by RefSeq, Jul 2008]

MLL2: may be a transcriptional regulator. Contains 3 A.T hook DNA-binding domains. Two alternatively spliced isoforms have been described. Protein type: Methyltransferase; EC 2.1.1.43; Transcription factor; Methyltransferase, protein lysine; DNA-binding. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: nucleoplasm; histone methyltransferase complex; nucleus. Molecular Function: protein binding; DNA binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); transcription factor activity. Biological Process: ovulation; establishment and/or maintenance of chromatin architecture; ovarian follicle development; transcription, DNA-dependent; chromatin-mediated maintenance of transcription; histone H3-K4 methylation; oocyte differentiation; regulation of histone H3-K4 methylation; gene silencing

0.05 mg

530 USD