Goat Polyclonal to Human P4HA1 | MyBioSource MBS242305 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat Polyclonal to Human P4HA1

catalog :

MBS242305

quantity :

0.05 mg

price :

495 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, dog, chicken, cow

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS242305

products type :

Antibody

products full name :

Goat Polyclonal to Human P4HA1

products short name :

P4HA1

products name syn :

Anti-P4HA1 Antibody (Internal) IHC-plus; P4HA1; 4-PH alpha-1; C-P4Halpha(I); p4HA; p4HA1; Human P4HA1

other names :

prolyl 4-hydroxylase subunit alpha-1 isoform 1; Prolyl 4-hydroxylase subunit alpha-1; prolyl 4-hydroxylase subunit alpha-1; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I; prolyl 4-hydroxylase, alpha polypeptide I; Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1

products gene name :

P4HA1

other gene names :

P4HA1; P4HA1; P4HA; P4HA; 4-PH alpha-1

uniprot entry name :

P4HA1_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)

sequence length :

534

specificity :

Human P4HA1. This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1).

purity :

Immunoaffinity Purified

form :

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

concentration :

0.5 mg/ml

storage stability :

Store at -20 degree C. Minimize freezing and thawing.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

app notes :

Immunohistochemistry: It was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml.

other info1 :

Target Species: Human. Immunogen: P4HA1 antibody was raised against synthetic peptide C-DFARKDEPDAFKE from an internal region of human P4HA1 (NP_000908.2; NP_001017962.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Lizard (100%); Mouse, Rat, Hamster, Opossum, Salmon, Stickleback, Pufferfish, Zebrafish (92%).

other info2 :

Immunogen Type: Synthetic peptide

ncbi gi num :

63252886

ncbi acc num :

NP_000908.2

ncbi gb acc num :

NM_000917.3

uniprot acc num :

P13674

ncbi mol weight :

58,958 Da

ncbi pathways :

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Metabolic Pathways (132956)

ncbi summary :

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

P4HA1: Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. Belongs to the P4HA family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Mitochondrial; Oxidoreductase; EC 1.14.11.2; Endoplasmic reticulum; Amino Acid Metabolism - arginine and proline. Chromosomal Location of Human Ortholog: 10q22.1. Cellular Component: intracellular membrane-bound organelle; membrane; mitochondrion; endoplasmic reticulum; endoplasmic reticulum lumen. Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding; procollagen-proline 4-dioxygenase activity; L-ascorbic acid binding; iron ion binding. Biological Process: collagen fibril organization; peptidyl-proline hydroxylation to 4-hydroxy-L-proline

size1 :

0.05 mg

price1 :

495 USD

more info or order :

MyBioSource product webpage