antibody

Goat Polyclonal to Human NRAS / N-ras

MBS242079

0.05 mg

495 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Goat Polyclonal to Human NRAS / N-ras

NRAS / N-ras

Anti-NRAS / N-ras Antibody (Internal) IHC-plus; NRAS; HRAS1; NRAS1; NS6; ALPS4; Transforming protein N-Ras; N-ras; N-ras protein part 4; GTPase NRas; Human NRAS; N-ras

GTPase NRas; GTPase NRas; GTPase NRas; N-ras protein part 4; transforming protein N-Ras; v-ras neuroblastoma RAS viral oncogene homolog; neuroblastoma RAS viral (v-ras) oncogene homolog; Transforming protein N-Ras

NRAS

N-ras

NRAS; NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1; HRAS1

RASN_HUMAN

Polyclonal

Goat

Reactivity: . Human, Mouse, Bat, Bovine, Dog, Horse, Pig (tested or 100% immunogen sequence identity). Predicted Reactivity: . Monkey, Rat, Guinea pig, Hamster (at least 90% immunogen sequence identity)

189

Human NRAS.

Immunoaffinity Purified

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

0.5 mg/ml

Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

Usage Summary: . Immunohistochemistry: MBS242079 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS242079 was determined to be 2.5 ug/ml. Uses:. IHC - Paraffin (2.5 µg/ml), Western blot (1:32000) & (0.01 - 0.03 ug/ml), ELISA (1:32000) (Optimal dilution to be determined by the researcher).

Target Species: Human. Immunogen Description: Synthetic peptide C-RMKKLNSSDDGTQ from an internal region of human NRAS (NP_002515.1). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum (100%).

Immunogen Type: NRAS / N-ras antibody was raised against synthetic peptide C-RMKKLNSSDDGTQ from an internal region of human NRAS (NP_002515.1). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Mouse, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum (100%); Marmoset, Rat, Hamster, Guinea pig (92%). Antigen Modification: Internal

Family: Ras GTPase superfamily IPR001806. Subfamily: RAS oncogene

The N-ras oncogene is a member of the RAS gene family. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. The order of nearby genes is as follows: cen--CD2--NGFB--NRAS--tel. The mammalian ras gene family consists of the harvey and kirsten ras genes (c-Hras1 and c-Kras2), an inactive pseudogene of each (c-Hras2 and c-Kras1) and the N-ras gene. They differ significantly only in the C-terminal 40 amino acids. These ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. Mutations which change amino acid residues 12, 13 or 61 activate the potential of N-ras to transform cultured cells and are implicated in a variety of human tumors. The N-ras gene specifies two main transcripts of 2Kb and 4.3Kb. The difference between the two transcripts is a simple extension through the termination site of the 2Kb transcript. The N-ras gene consists of seven exons (-I, I, II, III, IV, V, VI). The smaller 2Kb transcript contains the VIa exon, and the larger 4.3Kb transcript contains the VIb exon which is just a longer form of the VIa exon. Both transcripts encode identical proteins as they differ only the 3' untranslated region. The sequence of the shorter 2Kb transcript is presented here. The 4.3 Kb transcript sequence is not available.

4505451

NP_002515.1

NM_002524.4

P01111

ARMS-mediated Activation Pathway (106466); Activation Of RAS In B Cells Pathway (576252); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (366160); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688)

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]

NRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. Protein type: G protein, monomeric; G protein, monomeric, Ras; Motility/polarity/chemotaxis; Oncoprotein; G protein. Chromosomal Location of Human Ortholog: 1p13.2. Cellular Component: Golgi membrane; membrane; plasma membrane. Molecular Function: GTP binding; protein complex binding. Biological Process: regulation of long-term neuronal synaptic plasticity; epidermal growth factor receptor signaling pathway; axon guidance; negative regulation of skeletal muscle development; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; activation of MAPKK activity; MAPKKK cascade; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; small GTPase mediated signal transduction; Ras protein signal transduction; positive regulation of cell proliferation; insulin receptor signaling pathway; striated muscle cell differentiation; visual learning; innate immune response; negative regulation of neuron apoptosis; actin cytoskeleton organization and biogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration. Disease: Thyroid Carcinoma, Follicular; Ras-associated Autoimmune Leukoproliferative Disorder; Noonan Syndrome 6; Schimmelpenning-feuerstein-mims Syndrome; Melanocytic Nevus Syndrome, Congenital; Colorectal Cancer; Melanosis, Neurocutaneous; Nevus, Epidermal

0.05 mg

495 USD