catalog number :
MBS242056
products full name :
Goat Polyclonal to Human PC / Pyruvate Carboxylase
products short name :
PC / Pyruvate Carboxylase
products name syn :
Anti-PC / Pyruvate Carboxylase Antibody (Internal) IHC-plus; PC; PCB; Pyruvate carboxylase; Pyruvic carboxylase; Human PC; Pyruvate Carboxylase
other names :
pyruvate carboxylase, mitochondrial; Pyruvate carboxylase, mitochondrial; pyruvate carboxylase, mitochondrial; pyruvic carboxylase; pyruvate carboxylase; Pyruvic carboxylase; PCB
other gene names :
PC; PC; PCB; PCB
uniprot entry name :
PYC_HUMAN
reactivity :
Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat. Predicted Reactivity: Goat (at least 90% immunogen sequence identity)
specificity :
Human PC / Pyruvate Carboxylase. All reported variants (NP_000911.2; NP_001035806.1; NP_071504.2) represent identical protein.
purity :
Immunoaffinity Purified
form :
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
concentration :
0.5 mg/ml
storage stability :
Store at -20 degree C. Minimize freezing and thawing.
tested application :
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
app notes :
ELISA (1:16000), IHC-P (5 ug/ml), WB (0.03 - 0.1 ug/ml). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.
other info1 :
Target Species: Human. Immunogen Description: Synthetic peptide C-KFKEVKKAYVEANQ from an internal region of human PC / Pyruvate Carboxylase (NP_000911.2; NP_001035806.1; NP_071504.2).
other info2 :
Immunogen Type: Synthetic peptide. Immunogen: PC / Pyruvate Carboxylase antibody was raised against synthetic peptide C-KFKEVKKAYVEANQ from an internal region of human PC / Pyruvate Carboxylase (NP_000911.2; NP_001035806.1; NP_071504.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%); Goat, Stickleback, Pufferfish (93%); Opossum, Chicken, Xenopus, Zebrafish (86%). Antigen Modification: Internal
ncbi acc num :
NP_000911.2
ncbi gb acc num :
NM_000920.3
ncbi mol weight :
57,925 Da
ncbi pathways :
Alanine And Aspartate Metabolism Pathway 198783!!Biosynthesis Of Amino Acids Pathway 790012!!Biosynthesis Of Amino Acids Pathway 795174!!Biotin Transport And Metabolism Pathway 106257!!Carbon Metabolism Pathway 814926!!Carbon Metabolism Pathway 817567!!Citrate Cycle (TCA Cycle) Pathway 82927!!Citrate Cycle (TCA Cycle) Pathway 288!!Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway 906000!!Defective BTD Causes Biotidinase Deficiency Pathway 906015
ncbi summary :
This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]