Rabbit Polyclonal to Human c-Maf | MyBioSource MBS241700 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal to Human c-Maf

catalog :

MBS241700

quantity :

0.05 mg

price :

495 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS241700

products type :

Antibody

products full name :

Rabbit Polyclonal to Human c-Maf

products short name :

c-Maf

products name syn :

Anti-c-Maf Antibody (aa50-100) IHC-plus; C-MAF; CCA4; C-maf proto-oncogene; Proto-oncogene c-Maf; T lymphocyte c-maf long form; Transcription factor Maf; c-Maf; Human c-Maf

other names :

transcription factor Maf isoform a; Transcription factor Maf; transcription factor Maf; c-maf proto-oncogene; proto-oncogene c-Maf; T lymphocyte c-maf long form; Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog; Proto-oncogene c-Maf; V-maf musculoaponeurotic fibrosarcoma oncogene homolog

products gene name :

MAF

other gene names :

MAF; MAF; CCA4; c-MAF

uniprot entry name :

MAF_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Gibbon, Bovine, Dog, Gorilla, Human, Monkey, Mouse, Rat, Zebrafish. Predicted Reactivity: Xenopus (at least 90% immunogen sequence identity)

sequence length :

403

specificity :

A portion of amino acids 50-100 of human c-maf

purity :

Immunoaffinity Purified

form :

PBS, 0.2% gelatin, 0.05% sodium azide.

concentration :

0.5 mg/ml

storage stability :

Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB)

app notes :

IHC-P (10 ug/ml), WB (2 - 4 ug/ml). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.

other info1 :

Target Species: Human. Immunogen Description: A synthetic peptide corresponding to amino acids 75-90 (APSPGSGSEQKAHLED) of human c-maf was used as the immunogen, GenBank no. NP_001026974.1 .

other info2 :

Immunogen Type: Synthetic peptide. Immunogen: c-Maf antibody was raised against a synthetic peptide corresponding to amino acids 75-90 (APSPGSGSEQKAHLED) of human c-maf was used as the immunogen, GenBank no. NP_001026974.1 . Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Stickleback, Pufferfish, Zebrafish (100%); Xenopus (94%); Opossum (88%); Chicken (81%). Antigen Modification: aa50-100

ncbi gi num :

5453736

ncbi acc num :

NP_005351.2

ncbi gb acc num :

NM_005360.4

uniprot acc num :

O75444

ncbi mol weight :

41,961 Da

ncbi pathways :

C-MYB Transcription Factor Network Pathway (138073); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797); Keap1-Nrf2 Pathway (198777); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

ncbi summary :

The protein encoded by this gene is a DNA-binding, leucine zipper-containing transcription factor that acts as a homodimer or as a heterodimer. Depending on the binding site and binding partner, the encoded protein can be a transcriptional activator or repressor. This protein plays a role in the regulation of several cellular processes, including embryonic lens fiber cell development, increased T-cell susceptibility to apoptosis, and chondrocyte terminal differentiation. Defects in this gene are a cause of juvenile-onset pulverulent cataract as well as congenital cerulean cataract 4 (CCA4). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

uniprot summary :

MAF: Acts as a transcriptional activator or repressor. Involved in embryonic lens fiber cell development. Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation. Activates the expression of IL4 in T helper 2 (Th2) cells. Increases T-cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, transactivates strongly the glucagon gene promoter through the G1 element. Activates transcription of the CD13 proximal promoter in endothelial cells. Represses transcription of the CD13 promoter in early stages of myelopoiesis by affecting the ETS1 and MYB cooperative interaction. Involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. Binds to the sequence 5 -[GT]G[GC]N[GT]NCTCAGNN-3 in the L7 promoter. Binds to the T-MARE (Maf response element) sites of lens-specific alpha- and beta-crystallin gene promoters. Binds element G1 on the glucagon promoter. Binds an AT-rich region adjacent to the TGC motif (atypical Maf response element) in the CD13 proximal promoter in endothelial cells. When overexpressed, represses anti-oxidant response element (ARE)- mediated transcription. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. Binds to the ARE sites of detoxifying enzyme gene promoters. Homodimer or heterodimer with other bHLH-Zip transcription factors. Binds DNA as a homodimer or as a heterodimer. Heterotetramer of two MAF and two USF2. Interacts with PAX6; the interaction is direct. Interacts with MYB; interaction takes place weakly in normal T-cells and increases in T-cells following stimulation through the TCR engagement. Interacts with MYB; the ternary complex formed with MYB and the CD13 promoter is regulated in response to differentiating signals. Interacts with USF2; the interaction inhibits its DNA-binding activity on the L7 promoter. Interacts with CREBBP, EP300 and ETS1. Up-regulated with tert-butyl hydroquinone (t-BHQ). Expressed in endothelial cells. Belongs to the bZIP family. Maf subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Tumor suppressor; Transcription factor; Oncoprotein; DNA-binding; Cell development/differentiation. Chromosomal Location of Human Ortholog: 16q22-q23. Cellular Component: cytoplasm; chromatin; nucleus. Molecular Function: sequence-specific DNA binding. Biological Process: transcription from RNA polymerase II promoter; cytokine production; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; regulation of chondrocyte differentiation; inner ear development; cell development. Disease: Cataract 21, Multiple Types; Ayme-gripp Syndrome

size1 :

0.05 mg

price1 :

495 USD

more info or order :

MyBioSource product webpage