antibody

Goat Polyclonal to Human NALP3 / NLRP3

MBS241660

0.05 mg

495 USD

polyclonal

goat

nonconjugated

ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Goat Polyclonal to Human NALP3 / NLRP3

NALP3 / NLRP3

Anti-NALP3 / NLRP3 Antibody (Internal) IHC-plus; NLRP3; AGTAVPRL; AII; C1orf7; Caterpiller protein 1.1; Cryopyrin; FCAS; FCU; PYPAF1; NALP3; CIAS1; CLR1.1; MWS; Human NALP3; NLRP3

NACHT, LRR and PYD domains-containing protein 3 isoform a; NACHT, LRR and PYD domains-containing protein 3; NACHT, LRR and PYD domains-containing protein 3; cryopyrin; caterpiller protein 1.1; PYRIN-containing APAF1-like protein 1; NACHT, LRR and PYD containing protein 3; cold autoinflammatory syndrome 1 protein; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; NLR family, pyrin domain containing 3; Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1; CLR1.1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; PYRIN-containing APAF1-like protein 1

NALP3

NLRP3

NLRP3; NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL; C1orf7; CIAS1; NALP3; PYPAF1; CLR1.1

NALP3_HUMAN

Polyclonal

Goat

Gibbon, Gorilla, Human, Monkey. Predicted Reactivity: Bat, Pig (at least 90% immunogen sequence identity)

1036

Human NLRP3. This antibody is expected to recognise all five reported isoforms (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).

Immunoaffinity Purified

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

0.5 mg/ml

Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC - Paraffin), ELISA (EIA)

ELISA (1:64000), IHC-P (2.5 ug/ml). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.

Target Species: Human. Immunogen Description: Synthetic peptide C-DLYEKAKRDEPK from an internal region of human NLRP3 (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%).

Immunogen Type: Synthetic peptide. Immunogen: NALP3 / NLRP3 antibody was raised against synthetic peptide C-DLYEKAKRDEPK from an internal region of human NLRP3 (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Bat, Panda, Pig (92%); Dog, Bovine, Rabbit (83%). Antigen Modification: Internal

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis.

34878693

NP_004886.3

NM_004895.4

Q96P20

115,968 Da

Immune System Pathway (106386); Inflammasomes Pathway (366166); Influenza A Pathway (217173); Influenza A Pathway (217150); Innate Immune System Pathway (106387); NOD Pathway (198792); NOD-like Receptor Signaling Pathway (122191); NOD-like Receptor Signaling Pathway (122131); Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways (366164); Pertussis Pathway (218111)

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Oct 2008]

NLRP3: May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1); also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS); also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA); also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. Belongs to the NLRP family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Inhibitor. Chromosomal Location of Human Ortholog: 1q44. Cellular Component: cytoplasm; cytosol. Molecular Function: peptidoglycan binding; protein binding; ATP binding. Biological Process: caspase activation; apoptosis; negative regulation of acute inflammatory response; interleukin-1 beta production; positive regulation of caspase activity; defense response; positive regulation of interleukin-1 beta secretion; negative regulation of NF-kappaB import into nucleus; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; negative regulation of interleukin-1 beta secretion; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; innate immune response; interleukin-18 production; interleukin-1 secretion; detection of biotic stimulus; inflammatory response; defense response to virus. Disease: Cinca Syndrome; Familial Cold Autoinflammatory Syndrome 1; Muckle-wells Syndrome

0.05 mg

495 USD