Rabbit Polyclonal (IgG) to Human NOS1 / nNOS | MyBioSource MBS241618 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal (IgG) to Human NOS1 / nNOS

catalog :

MBS241618

quantity :

0.1 mL

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, dog, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-nNOS / NOS1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

image 2 :

Anti-nNOS / NOS1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

image 3 :

Western blot of NNOS / NOS antibody.

product information

catalog number :

MBS241618

products type :

Antibody

products full name :

Rabbit Polyclonal (IgG) to Human NOS1 / nNOS

products short name :

[NOS1 / nNOS]

products name syn :

[Anti-NOS1 / nNOS Antibody (aa1422-1433) IHC-plus; NOS1; BNOS; Constitutive NOS; IHPS1; NNOS; NC-NOS; Nitric oxide synthase; brain; N-NOS; Neuronal NOS; NOS; NOS type I; Nos; type i; Human NOS1; nNOS]

other names :

[nitric oxide synthase, brain isoform 1; Nitric oxide synthase, brain; nitric oxide synthase, brain; NOS type I; neuronal NOS; constitutive NOS; peptidyl-cysteine S-nitrosylase NOS1; nitric oxide synthase 1 (neuronal); Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS]

products gene name :

[NOS1]

products gene name syn :

[nNOS]

other gene names :

[NOS1; NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS; N-NOS; nNOS]

uniprot entry name :

NOS1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Monkey, Mouse, Bat, Dog, Horse, Rabbit (tested or 100% immunogen sequence identity). Predicted Reactivity: Rat, Chicken (at least 90% immunogen sequence identity)

sequence length :

1434

specificity :

Human nNOS amino acids 1422-1433 (ESKKDTDEVFSS)1,2

purity :

Immunoaffinity Purified

form :

TBS, pH 7.4, containing 50% glycerol and 0.02% sodium azide

concentration :

0.4 mg/ml

storage stability :

Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), Immunoprecipitation (IP)

app notes :

ICC, IHC-P (5 ug/ml), IP, WB. Usage: Immunohistochemistry: MBS241618 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS241618 was determined to be 5 ug/ml.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Western Blot (WB)

other info1 :

Target: Human NOS1/nNOS. Target Species: Human. Immunogen Description: Human nNOS amino acids 1422-1433 (ESKKDTDEVFSS). Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Mouse, Dog, Bat, Elephant, Horse, Rabbit (100%); Rat, Chicken (92%); Xenopus, Zebrafish (83%).

other info2 :

Immunogen Species: NOS1 / nNOS antibody was raised against human. Immunogen: NOS1 / nNOS antibody was raised against human nNOS amino acids 1422-1433 (ESKKDTDEVFSS). Percent identity by BLAST analysis: Human, Gorilla, Marmoset, Mouse, Dog, Bat, Elephant, Horse, Rabbit (100%); Rat, Chicken (92%); Xenopus, Zebrafish (83%). Antigen Modification: aa1422-1433. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products description :

NOS1 / nNOS belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.

ncbi gi num :

10835173

ncbi acc num :

NP_000611.1

ncbi gb acc num :

NM_000620.4

uniprot acc num :

P29475

ncbi pathways :

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459)

ncbi summary :

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

uniprot summary :

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described. Protein type: Oxidoreductase; Amino Acid Metabolism - arginine and proline; EC 1.14.13.39. Chromosomal Location of Human Ortholog: 12q24.22. Cellular Component: protein complex; cytoskeleton; photoreceptor inner segment; mitochondrion; sarcoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; dendritic spine; synapse; cytosol; sarcolemma; lipid raft. Molecular Function: calmodulin binding; protein binding; FAD binding; sodium channel regulator activity; FMN binding; nitric-oxide synthase activity; NADPH-hemoprotein reductase activity; iron ion binding; heme binding; oxidoreductase activity; NADP binding; cadmium ion binding. Biological Process: negative regulation of serotonin uptake; interaction with host; negative regulation of calcium ion transport; striated muscle contraction; positive regulation of transcription, DNA-dependent; negative regulation of potassium ion transport; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; positive regulation of vasodilation; response to heat; negative regulation of hydrolase activity; exogenous drug catabolic process; response to hypoxia; peptidyl-cysteine S-nitrosylation; arginine catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; negative regulation of blood pressure; nitric oxide biosynthetic process; blood coagulation; multicellular organismal response to stress; nitric oxide mediated signal transduction; myoblast fusion; neurotransmitter biosynthetic process. Disease: Pyloric Stenosis, Infantile Hypertrophic, 1

size1 :

0.1 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage