antibody

Rabbit Polyclonal (IgG) to Rat MAPK1 / ERK2 / p42

MBS241457

0.05 mL

495 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, immunoprecipitation

Antibody

Rabbit Polyclonal (IgG) to Rat MAPK1 / ERK2 / p42

MAPK1 / ERK2 / p42

Anti-MAPK1 / ERK2 / p42 Antibody (C-Terminus) IHC-plus; MAPK1; ERK; ERK-2; ERK2; MAP kinase 1; MAPK 2; MAPK2; MAP kinase isoform p42; MAPK 1; p41mapk; PRKM2; MAP kinase 2; p41; p42-MAPK; PRKM1; ERT1; p38; p40; p42MAPK; Protein tyrosine kinase ERK2; Rat MAPK1; ERK2; p42

mitogen-activated protein kinase 1; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 1; MAPK 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; protein tyrosine kinase ERK2; mitogen-activated protein kinase 2; extracellular signal-regulated kinase 2; mitogen-activated protein kinase 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2

MAPK1

ERK2; p42

MAPK1; MAPK1; ERK; p38; p40; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK; ERK2; PRKM1; PRKM2; MAP kinase 1; MAPK 1; ERK-2; p42-MAPK; MAP kinase 2; MAPK 2

MK01_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

360

Peptide mapping to the carboxy terminus of rat ERK2

Affinity Purified

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol, 0.1% gelatin.

+4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP)

IHC-P (1:50), IP, WB. Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.

Target Species: Rat. Immunogen Description: Synthetic peptide from rat MAPK1 / ERK.

Immunogen Type: Synthetic peptide. Immunogen: MAPK1 / ERK2 / p42 antibody was raised against synthetic peptide from rat MAPK1 / ERK. Antigen Modification: C-Terminus

Family: Protein Kinase. Subfamily: MAPK

ERK2, a MAPK type protein kinase, is a member of the Ras MAPK pathway. This pathway modulates the activity of transcription factors, structural proteins and other protein kinases, leading to biological responses such as cell proliferation and differentiation. Inactive ERK2 associates with MAPK/ERK kinases (MEK) 1 and 2 in the cytosol. Upon stimulation, MEK1/2 phosphorylates and activates ERK2 causing dissociation of the MEK/ERK complex.

66932916

NP_002736.3

NM_002745.4

P28482

36,432 Da

AGE/RAGE Pathway (698754); ALK1 Signaling Events Pathway (137968); ARMS-mediated Activation Pathway (106466); ATF-2 Transcription Factor Network Pathway (138006); Activated TLR4 Signalling Pathway (106400); Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Activation Of The AP-1 Family Of Transcription Factors Pathway (160139); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adherens Junction Pathway (83070)

This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]

ERK2: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates. Protein type: EC 2.7.11.24; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; MAPK family; ERK subfamily; MAPK/ERK subfamily. Chromosomal Location of Human Ortholog: 22q11.21. Cellular Component: dendrite cytoplasm; Golgi apparatus; focal adhesion; protein complex; mitochondrion; early endosome; perikaryon; caveola; pseudopodium; cytosol; nucleoplasm; microtubule cytoskeleton; cytoskeleton; axon; cytoplasm; late endosome; microtubule organizing center; nucleus. Molecular Function: RNA polymerase subunit kinase activity; MAP kinase activity; protein serine/threonine kinase activity; protein binding; DNA binding; phosphotyrosine binding; mitogen-activated protein kinase kinase kinase binding; transcription factor binding; ATP binding; phosphatase binding. Biological Process: viral reproduction; activation of MAPKK activity; nerve growth factor receptor signaling pathway; activation of MAPK activity; response to toxin; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; sensory perception of pain; T cell receptor signaling pathway; protein amino acid phosphorylation; toll-like receptor 5 signaling pathway; B cell receptor signaling pathway; regulation of transcription factor activity; small GTPase mediated signal transduction; toll-like receptor 4 signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; transcription, DNA-dependent; toll-like receptor 2 signaling pathway; regulation of stress-activated MAPK cascade; nuclear translocation of MAPK; organ morphogenesis; response to estrogen stimulus; toll-like receptor 9 signaling pathway; regulation of cytoskeleton organization and biogenesis; axon guidance; positive regulation of translation; apoptosis; regulation of protein stability; chemotaxis; signal transduction; toll-like receptor 10 signaling pathway; synaptic transmission; response to exogenous dsRNA; positive regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; response to stress; epidermal growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; peptidyl-threonine phosphorylation; mammary gland epithelial cell proliferation; cell cycle; MyD88-dependent toll-like receptor signaling pathway; negative regulation of cell differentiation; peptidyl-serine phosphorylation; cytosine metabolic process; Ras protein signal transduction; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; blood coagulation; vascular endothelial growth factor receptor signaling pathway; response to DNA damage stimulus; positive regulation of cell migration

0.05 mL

495 USD