Goat Polyclonal to Human BMI1 / PCGF4 | MyBioSource MBS240598 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat Polyclonal to Human BMI1 / PCGF4

catalog :

MBS240598

quantity :

0.05 mg

price :

495 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS240598

products type :

Antibody

products full name :

Goat Polyclonal to Human BMI1 / PCGF4

products short name :

BMI1 / PCGF4

products name syn :

Anti-BMI1 / PCGF4 Antibody (aa252-264) IHC-plus; BMI1; BMI-1; Flvi-2/bmi-1; FLVI2/BMI1; PCGF4; Polycomb group protein Bmi1; Polycomb complex protein BMI-1; Dna-binding protein bmi-1; Polycomb group ring finger 4; RING finger protein 51; RNF51; Human BMI1; PCGF4

other names :

polycomb complex protein BMI-1; Polycomb complex protein BMI-1; polycomb complex protein BMI-1; flvi-2/bmi-1; ring finger protein 51; polycomb group protein Bmi1; BMI1 polycomb ring finger oncogene; polycomb group RING finger protein 4; BMI1 polycomb ring finger proto-oncogene; B lymphoma Mo-MLV insertion region 1 homolog; murine leukemia viral (bmi-1) oncogene homolog; BMI1 proto-oncogene, polycomb ring finger; Polycomb group RING finger protein 4; RING finger protein 51

products gene name :

BMI1

products gene name syn :

PCGF4

other gene names :

BMI1; BMI1; PCGF4; RNF51; FLVI2/BMI1; PCGF4; RNF51

uniprot entry name :

BMI1_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Human

sequence length :

326

specificity :

Amino acids 252-264 of human Bmi1 protein.

purity :

Immunoaffinity Purified

form :

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.

concentration :

0.91 mg/ml

storage stability :

Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

tested application :

Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)

app notes :

ELISA (1:5000 - 1:30000), ICC, IF, IHC-P (2.5 ug/ml), WB (1:500 - 1:3000). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.

other info1 :

Target Species: Human. Immunogen Description: Synthetic peptide from human BMI1.

other info2 :

Immunogen Type: Synthetic peptide. Immunogen: BMI1 / PCGF4 antibody was raised against synthetic peptide from human BMI1. Antigen Modification: aa252-264

ncbi gi num :

27883842

ncbi acc num :

NP_005171.4

ncbi gb acc num :

NM_005180.8

uniprot acc num :

P35226

ncbi mol weight :

36,949 Da

ncbi pathways :

Cellular Senescence Pathway (905991); Cellular Responses To Stress Pathway (645258); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); Oxidative Stress Induced Senescence Pathway (905993); Senescence And Autophagy Pathway (198780); Signaling Pathways Regulating Pluripotency Of Stem Cells (1026136); Signaling Pathways Regulating Pluripotency Of Stem Cells (1033502); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

ncbi summary :

This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015]

uniprot summary :

BMI1: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A Lys-119 , rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. Component of a PRC1-like complex. Interacts with RING1 and RING2. Interacts vwith CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1. Protein type: Motility/polarity/chemotaxis; EC 6.3.2.-; Ubiquitin ligase; Oncoprotein; Ubiquitin conjugating system; Ligase. Chromosomal Location of Human Ortholog: 10p11.23. Cellular Component: nucleoplasm; heterochromatin; nuclear body; cytoplasm; PcG protein complex; nucleus; ubiquitin ligase complex. Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; ubiquitin-protein ligase activity; chromatin binding. Biological Process: transcription, DNA-dependent; in utero embryonic development; positive regulation of immature T cell proliferation in the thymus; negative regulation of transcription from RNA polymerase II promoter; humoral immune response; embryonic skeletal morphogenesis; segment specification; rostrocaudal neural tube patterning; positive regulation of fibroblast proliferation; regulation of gene expression; positive regulation of ubiquitin-protein ligase activity; histone ubiquitination; DNA methylation; positive regulation of B cell proliferation; somatic stem cell division; hemopoiesis; brain development; histone acetylation

size1 :

0.05 mg

price1 :

495 USD

more info or order :

MyBioSource product webpage