antibody

Rabbit Polyclonal to Human CDK5

MBS240590

0.05 mL

495 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, immunoprecipitation, enzyme immunoassay

Antibody

Rabbit Polyclonal to Human CDK5

CDK5

Anti-CDK5 Antibody (C-Terminus) IHC-plus; CDK5; CDKN5; Cell division protein kinase 5; Crk6; Cyclin-dependent kinase 5; Protein kinase CDK5 splicing; PSSALRE; TPKII catalytic subunit; Human CDK5

cyclin-dependent-like kinase 5 isoform 1; Cyclin-dependent-like kinase 5; cyclin-dependent-like kinase 5; TPKII catalytic subunit; protein kinase CDK5 splicing; cell division protein kinase 5; serine/threonine-protein kinase PSSALRE; tau protein kinase II catalytic subunit; cyclin-dependent kinase 5; Cell division protein kinase 5; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit

CDK5

CDK5; CDK5; PSSALRE; CDKN5; TPKII catalytic subunit

CDK5_HUMAN

Polyclonal

Rabbit

Human, Mouse, Rat

292

Cdk5 (p31) peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Antiserum

0.01% sodium azide.

85 mg/ml

Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)

ELISA (1:5000 - 1:20000), IHC-P (1:500), IP (1:100), WB (1:500 - 1:5000). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.

Target Species: Human. Target: CDK5

Antigen Modification: C-Terminus

Family: Protein Kinase. Subfamily: CDC2/CDK

Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Interacts with D1 and D3-type G1 cyclins. Phosphorylates SRC, NOS3, VIM/vimentin, p35/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM/MUC18, SEPT5, SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP/NFAT5, MAPT/TAU, MAP1B, histone H1, p53/TP53, HDAC1, APEX1, PTK2/FAK1, huntingtin/HTT, ATM, MAP2, NEFH and NEFM. Regulates several neuronal development and physiological processes including neuronal survival, migration and differentiation, axonal and neurite growth, synaptogenesis, oligodendrocyte differentiation, synaptic plasticity and neurotransmission, by phosphorylating key proteins. Activated by interaction with CDK5R1 (p35) and CDK5R2 (p39), especially in post-mitotic neurons, and promotes CDK5R1 (p35) expression in an autostimulation loop. Phosphorylates many downstream substrates such as Rho and Ras family small GTPases (e.g. PAK1, RAC1, RHOA, CDC42) or microtubule-binding proteins (e.g. MAPT/TAU, MAP2, MAP1B), and modulates actin dynamics to regulate neurite growth and/or spine morphogenesis. Phosphorylates also exocytosis associated proteins such as MCAM/MUC18, SEPT5, SYN1, and CDK16/PCTAIRE1 as well as endocytosis associated proteins such as DNM1, AMPH and SYNJ1 at synaptic terminals. In the mature central nervous system (CNS), regulates neurotransmitter movements by phosphorylating substrates associated with neurotransmitter release and synapse plasticity; synaptic vesicle exocytosis, vesicles fusion with the presynaptic membrane, and endocytosis. Promotes cell survival by activating anti-apoptotic proteins BCL2 and STAT3, and negatively regulating of JNK3/MAPK10 activity. Phosphorylation of p53/TP53 in response to genotoxic and oxidative stresses enhances its stabilization by preventing ubiquitin ligase-mediated proteasomal degradation, and induces transactivation of p53/TP53 target genes, thus regulating apoptosis. Phosphorylation of p35/CDK5R1 enhances its stabilization by preventing calpain-mediated proteolysis producing p25/CDK5R1 and avoiding ubiquitin ligase-mediated proteasomal degradation. During aberrant cell-cycle activity and DNA damage, p25/CDK5 activity elicits cell-cycle activity and double-strand DNA breaks that precedes neuronal death by deregulating HDAC1. DNA damage triggered phosphorylation of huntingtin/HTT in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. Phosphorylation of PXN reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Negative regulator of Wnt/beta-catenin signaling pathway. Activator of the GAIT (IFN-gamma-activated inhibitor of translation) pathway, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells; phosphorylates the linker domain of glutamyl-prolyl tRNA synthetase (EPRS) in a IFN-gamma-dependent manner, the initial event in assembly of the GAIT complex. Phosphorylation of SH3GLB1 is required for autophagy induction in starved neurons. Phosphorylation of TONEBP/NFAT5 in response to osmotic stress mediates its rapid nuclear localization. MEF2 is inactivated by phosphorylation in nucleus in response to neurotoxin, thus leading to neuronal apoptosis. APEX1 AP-endodeoxyribonuclease is repressed by phosphorylation, resulting in accumulation of DNA damage and contributing to neuronal death. NOS3 phosphorylation down regulates NOS3-derived nitrite (NO) levels. SRC phosphorylation mediates its ubiquitin-dependent degradation and thus leads to cytoskeletal reorganization. May regulate endothelial cell migration and angiogenesis via the modulation of lamellipodia formation. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution.

4826675

NP_004926.1

NM_004935.3

Q00535

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); BDNF Signaling Pathway (712093); CRMPs In Sema3A Signaling Pathway (119525); Cocaine Addiction Pathway (546258); Cocaine Addiction Pathway (546273)

This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]

CDK5: a protein kinase of the CDK family. Unlike other members of this family, it is not activated by cyclins but by p35 (CDK5R1) and p39. An important regulator of neuronal positioning during brain development. May also play a role in synaptogenesis and neurotransmission. Substrates include TAU, MAP2, NF-H and -M, Nudel, PDE6, beta-catenin, amphyphysin, dynamin I, synapsin 1, Munc-18, and NMDA receptor 2A. Plays a role in myogenesis, haematopoietic cell differentiation, spermatogenesis, insulin secretion, and lens differentiation. Implicated in the pathology of neurofibrillary tangles and formation of senile plaques, hallmarks of Alzheimer?s disease. Induces tau phosphorylation and aggregation and neurofibrillary tangle deposition and neurodegeneration in in vitro and in vivo animal models. Brain samples from Alzeimer?s pateints show elevated CDK5 activity. Protein type: Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, CMGC; Kinase, protein; CMGC group; CDK family; CDK5 subfamily; CDK/CDK5 subfamily. Chromosomal Location of Human Ortholog: 7q36. Cellular Component: cyclin-dependent protein kinase 5 activator complex; dendrite; postsynaptic density; perikaryon; cytosol; postsynaptic membrane; growth cone; cytoskeleton; cell soma; membrane; axon; lamellipodium; cytoplasm; cell junction; neuromuscular junction; nucleus; filopodium. Molecular Function: acetylcholine receptor activator activity; ephrin receptor binding; p53 binding; protein kinase activity; protein serine/threonine kinase activity; ErbB-2 class receptor binding; protein binding; ErbB-3 class receptor binding; cyclin-dependent protein kinase activity; cytoskeletal protein binding; tau-protein kinase activity; kinase activity; ATP binding. Biological Process: Schwann cell development; negative regulation of synaptic plasticity; axon extension; cerebellar cortex formation; rhythmic process; regulation of synaptic plasticity; motor axon guidance; sensory perception of pain; regulation of cell migration; receptor clustering; corpus callosum development; regulation of apoptosis; neuron differentiation; receptor catabolic process; oligodendrocyte differentiation; neurite development; central nervous system neuron development; synaptic transmission, glutamatergic; skeletal muscle development; dendrite morphogenesis; cell division; synaptic vesicle endocytosis; negative regulation of protein ubiquitination; negative regulation of transcription, DNA-dependent; regulated secretory pathway; synaptic transmission, dopaminergic; axon guidance; negative regulation of protein export from nucleus; positive regulation of protein binding; cell-matrix adhesion; protein amino acid autophosphorylation; neuron migration; nucleocytoplasmic transport; negative regulation of axon extension; negative regulation of cell cycle; synaptic transmission; intracellular protein transport; synaptogenesis; behavioral response to cocaine; positive regulation of neuron apoptosis; visual learning; cortical actin cytoskeleton organization and biogenesis; layer formation in the cerebral cortex; serine phosphorylation of STAT3 protein; negative regulation of proteolysis; hippocampus development; peptidyl-threonine phosphorylation; cell cycle; positive regulation of calcium ion-dependent exocytosis; cell proliferation; synaptic vesicle exocytosis; embryonic development; peptidyl-serine phosphorylation; neuron apoptosis; positive regulation of protein kinase activity; blood coagulation; regulation of excitatory postsynaptic membrane potential; phosphorylation. Disease: Lissencephaly 7 With Cerebellar Hypoplasia

0.05 mL

495 USD