antibody

Rabbit Polyclonal to Human DAXX

MBS240579

0.05 mg

495 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

Rabbit Polyclonal to Human DAXX

DAXX

Anti-DAXX Antibody (aa261-274) IHC-plus; DAXX; BING2; CENP-C binding protein; DAP6; Death-associated protein 6; EAP1; Fas-binding protein; HDaxx; ETS1-associated protein 1; Human DAXX

death domain-associated protein 6 isoform a; Death domain-associated protein 6; death domain-associated protein 6; Fas-binding protein; CENP-C binding protein; ETS1-associated protein 1; death-associated protein 6; fas death domain-associated protein; death-domain associated protein; Daxx; hDaxx; ETS1-associated protein 1; EAP1; Fas death domain-associated protein

DAXX

DAXX; DAXX; DAP6; EAP1; BING2; BING2; DAP6; hDaxx; EAP1

DAXX_HUMAN

Polyclonal

Rabbit

Human, Mouse, Rat

740

Amino acids 261-274 of human DAXX protein.

Immunoaffinity Purified

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.

1 mg/ml

+4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)

ELISA (1:7000 - 1:45000), IF, IHC-P (2.5 ug/ml), WB (1:200 - 1:2000). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.

Target Species: Human. Immunogen Description: Synthetic peptide from human Daxx.

Immunogen Type: Synthetic peptide. Immunogen: DAXX antibody was raised against synthetic peptide from human Daxx. Antigen Modification: aa261-274

Family: Apoptosis

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family.

4503257

NP_001341.1

NM_001350.4

Q9UER7

73,589 Da

Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Androgen Receptor Signaling Pathway (198806); Apoptosis Modulation And Signaling Pathway (198822); FAS Pathway And Stress Induction Of HSP Regulation (198894); HIV-1 Nef: Negative Effector Of Fas And TNF-alpha Pathway (137995); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); IL-6 Signaling Pathway (198864)

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

DAXX: a transcriptional co-regulatory protein. Proposed to mediate activation of the JNK pathway and apoptosis via ASK1 in response to signaling from FAS and TGF-betaR2. Glucose deprivation activates the ASK1-SEK1-JNK1-HIPK1 pathway, relocalizing Daxx from the nucleus to the cytoplasm, where Daxx binds to ASK1, and subsequently leads to ASK1 oligomerization. Interaction with HSP27 may prevent interaction with TGF-betaR2 and ASK1 and block DAXX-mediated apoptosis. Seems to act as a transcriptional co- repressor and inhibits PAX3 and ETS1 through direct protein- protein interaction. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Two alternatively spliced isoforms have been described. Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Apoptosis; Nucleolus. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: heterochromatin; PML body; cytoplasm; nucleolus; nucleus; cytosol; chromosome, pericentric region. Molecular Function: protein homodimerization activity; histone binding; p53 binding; protein N-terminus binding; protein kinase binding; transcription factor binding; protein binding; enzyme binding; androgen receptor binding; ubiquitin protein ligase binding; heat shock protein binding; receptor signaling protein activity; transcription corepressor activity; protein kinase activator activity. Biological Process: transcription, DNA-dependent; viral reproduction; apoptosis; regulation of protein ubiquitination; activation of JNK activity; chromatin remodeling; nucleosome assembly; induction of apoptosis via death domain receptors; cytokinesis after mitosis; regulation of transcription, DNA-dependent; positive regulation of protein kinase activity; androgen receptor signaling pathway; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent

0.05 mg

495 USD