Rabbit Polyclonal to Human PCNA / Cyclin | MyBioSource MBS240535 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal to Human PCNA / Cyclin

catalog :

MBS240535

quantity :

0.05 mg

price :

495 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, chicken, cow

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig s7a	Hasanov Z, Ruckdeschel T, König C, Mogler C, Kapel S, Korn C, et al. Endosialin Promotes Atherosclerosis Through Phenotypic Remodeling of Vascular Smooth Muscle Cells. Arterioscler Thromb Vasc Biol. 2017;37:495-505 pubmed publisher

product information

catalog number :

MBS240535

products type :

Antibody

products full name :

Rabbit Polyclonal to Human PCNA / Cyclin

products short name :

PCNA / Cyclin

products name syn :

Anti-PCNA / Cyclin Antibody (Internal) IHC-plus; Cyclin; Human PCNA; Cyclin

other names :

proliferating cell nuclear antigen; Proliferating cell nuclear antigen; proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein; proliferating cell nuclear antigen; Cyclin

products gene name :

PCNA

other gene names :

PCNA; PCNA; ATLD2; PCNA

uniprot entry name :

PCNA_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Bovine, Chicken, Dog, Fish, Xenopus, Human, Monkey, Mouse, Rat

sequence length :

261

specificity :

An internal region of human PCNA protein.

purity :

Immunoaffinity Purified

form :

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.

concentration :

1 mg/ml

storage stability :

+4 degree C or -20 degree C, Avoid repeated freezing and thawing.

tested application :

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

app notes :

ELISA (1:2000 - 1:8000), IHC-P (2.5 ug/ml), WB (1:500 - 1:2000). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.

other info1 :

Target Species: Human. Immunogen Description: Synthetic peptide from human PCNA.

other info2 :

Immunogen Type: Synthetic peptide. Immunogen: PCNA / Cyclin antibody was raised against synthetic peptide from human PCNA. Antigen Modification: Internal

ncbi gi num :

4505641

ncbi acc num :

NP_002583.1

ncbi gb acc num :

NM_002592.2

uniprot acc num :

P12004

ncbi mol weight :

28,769 Da

ncbi pathways :

BARD1 Signaling Events Pathway (137959); BRCA1-associated Genome Surveillance Complex (BASC) Pathway (413428); BRCA1-associated Genome Surveillance Complex (BASC) Pathway (890555); Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054)

ncbi summary :

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

uniprot summary :

PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase s processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3 - 5 exonuclease and 3 -phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family. Protein type: Cell cycle regulation; DNA replication. Chromosomal Location of Human Ortholog: 20pter-p12. Cellular Component: nucleoplasm; centrosome; PCNA complex; cytoplasm; nuclear replication fork; DNA replication factor C complex; nucleus. Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; MutLalpha complex binding; purine-specific mismatch base pair DNA N-glycosylase activity; dinucleotide insertion or deletion binding; receptor tyrosine kinase binding. Biological Process: mismatch repair; heart development; positive regulation of deoxyribonuclease activity; DNA strand elongation during DNA replication; leading strand elongation; response to lipid; DNA repair; telomere maintenance via semi-conservative replication; G1/S-specific transcription in mitotic cell cycle; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; base-excision repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; telomere maintenance; regulation of DNA replication; G1/S transition of mitotic cell cycle. Disease: Ataxia-telangiectasia-like Disorder 2

size1 :

0.05 mg

price1 :

495 USD

more info or order :

MyBioSource product webpage