Rabbit Polyclonal to Human SAE1 | MyBioSource MBS240515 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Polyclonal to Human SAE1

catalog :

MBS240515

quantity :

0.05 mg

price :

495 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS240515

products type :

Antibody

products full name :

Rabbit Polyclonal to Human SAE1

products short name :

SAE1

products name syn :

Anti-SAE1 Antibody IHC-plus; SAE1; Activator of SUMO1; AOS1; UBLE1A; HSPC140; SUA1; Human SAE1

other names :

SUMO-activating enzyme subunit 1 isoform a; SUMO-activating enzyme subunit 1; SUMO-activating enzyme subunit 1; activator of SUMO1; SUMO-1 activating enzyme subunit 1; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme; SUMO1 activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1ASUMO-activating enzyme subunit 1, N-terminally processed

products gene name :

SAE1

other gene names :

SAE1; SAE1; AOS1; SUA1; UBLE1A; HSPC140; AOS1; SUA1; UBLE1A

uniprot entry name :

SAE1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human

sequence length :

346

specificity :

Recombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding to full length Human SUMO Activating Enzyme E1 fused with GST.

purity :

Protein A Purified

form :

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.

concentration :

1 mg/ml

storage stability :

Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)

app notes :

ELISA (1:5000 - 1:20000), IF, IHC-P (5 ug/ml), WB (1:500 - 1:2000). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.

other info1 :

Target Species: Human. Resuspension Liquid Description: After adding water, will consist of 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide. Immunogen Description: Recombinant human SAE1.

other info2 :

Immunogen Type: Recombinant protein. Immunogen: SAE1 antibody was raised against recombinant human SAE1.

ncbi gi num :

4885585

ncbi acc num :

NP_005491.1

ncbi gb acc num :

NM_005500.2

uniprot acc num :

Q9UBE0

ncbi mol weight :

33,063 Da

ncbi pathways :

Metabolism Of Proteins Pathway (106230); Post-translational Protein Modification Pathway (161012); Processing And Activation Of SUMO Pathway (833806); SUMO Is Conjugated To E1 (UBA2:SAE1) Pathway (833808); SUMO Is Transferred From E1 To E2 (UBE2I, UBC9) Pathway (833809); SUMOylation Pathway (833805); Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)

ncbi summary :

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

uniprot summary :

UBLE1A: The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Belongs to the ubiquitin-activating E1 family. Protein type: Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 19q13.32. Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytosol; nucleus. Molecular Function: protein C-terminus binding; ATP-dependent protein binding; SUMO activating enzyme activity; protein binding; protein heterodimerization activity; enzyme activator activity; ubiquitin activating enzyme activity. Biological Process: positive regulation of catalytic activity; protein sumoylation; cellular protein metabolic process; regulation of mitotic cell cycle; protein ubiquitination; SMT3-dependent protein catabolic process; post-translational protein modification

size1 :

0.05 mg

price1 :

495 USD

more info or order :

MyBioSource product webpage