antibody

Rabbit Polyclonal to Human IL23A / IL-23 p19

MBS240347

0.05 mg

530 USD

polyclonal

rabbit

nonconjugated

human, mouse

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Rabbit Polyclonal to Human IL23A / IL-23 p19

[IL23A / IL-23 p19]

[Anti-IL23A / IL-23 p19 Antibody (N-Terminus) IHC-plus; IL23A; IL-23 subunit alpha; IL-23A; Interleukin 23 p19 subunit; Interleukin-23 subunit alpha; IL23P19; Interleukin-23 subunit p19; p19; SGRF; IL-23; IL-23 p19; IL-23-A; IL-23p19; Human IL23A; IL-23 p19]

[interleukin-23 subunit alpha; Interleukin-23 subunit alpha; interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin 23 p19 subunit; interleukin-23 subunit p19; JKA3 induced upon T-cell activation; interleukin-six, G-CSF related factor; interleukin 23, alpha subunit p19; Interleukin-23 subunit p19; IL-23p19]

[IL23A]

[IL-23 p19]

[IL23A; IL23A; P19; SGRF; IL-23; IL-23A; IL23P19; SGRF; IL-23 subunit alpha; IL-23-A; IL-23p19]

IL23A_HUMAN

polyclonal

IgG

rabbit

Human, Mouse

189

16 amino acid peptide from near the amino terminus of human IL-23

Peptide Column Affinity Chromatography

PBS containing 0.02% sodium azide.

1 mg/ml

Store at 4°C for up to 3 months. store at -20°C for up to 1 year. Avoid repeated freeze/ thaw cycles.

Immunohistochemistry (IHC - Paraffin), Immunohistochemistry (IHC), Western Blot (WB), ELISA (EIA)

Immunohistochemistry-Paraffin (IHC-P) (2.5 ug/ml). Western Blot (WB) (1 - 2 ug/ml). Usage: Immunohistochemistry: MBS240347 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary atnibody slides were incuated with biotinylated secondary antibody, followed by alkaline phophatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specifity. The optimal working concentration for MBS240347 was determined to be 2.5 ug/ml.

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunofluorescence (IF)

Western Blot (WB)

Western blot of IL-23 in mouse pancreas tissue lysate with IL-23 antibody at (A) 1 and (B) 2 ug/ml.

Target Species: IL23A/IL-23 p19. Immunogen Species: IL23A/IL-23 p19 antibody was raised against Human. Antigen Type: Synthetic Peptide. Usage: IL-23 antibody can be used for the detection of IL-23 by Western blot at 1 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 2 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 21 kDa Observed: 23 kDa

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip. Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.

Family: Interleukin

IL23 p19 (IL23A) is a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of the IL23A subunit and the p40 subunit of interleukin 12 (IL12B). Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naïve CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells.

7706702

NP_057668.1

NM_016584.2

Q9NPF7

ATF-2 Transcription Factor Network Pathway (138006); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL23-mediated Signaling Events Pathway (138000); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Pertussis Pathway (218111); Pertussis Pathway (218099)

This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells. [provided by RefSeq, Jul 2008]

IL23A: Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak- Stat signaling cascade, stimulates memory rather than naive T- cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. Belongs to the IL-6 superfamily. Protein type: Cytokine; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 12q13.3. Molecular Function: protein binding; interleukin-23 receptor binding; cytokine activity. Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; negative regulation of interleukin-10 production; positive regulation of T-helper 1 type immune response; positive regulation of T cell mediated cytotoxicity; positive regulation of osteoclast differentiation; positive regulation of interleukin-12 production; tissue remodeling; positive regulation of NK T cell proliferation; positive regulation of tyrosine phosphorylation of Stat4 protein; positive regulation of NF-kappaB import into nucleus; positive regulation of activated T cell proliferation; defense response to Gram-negative bacterium; positive regulation of natural killer cell activation; positive regulation of interleukin-10 production; positive regulation of tyrosine phosphorylation of Stat3 protein; T cell proliferation; positive regulation of T cell proliferation; inflammatory response; defense response to virus; positive regulation of interleukin-17 production; positive regulation of memory T cell differentiation; positive regulation of natural killer cell proliferation; positive regulation of NK T cell activation; positive regulation of tumor necrosis factor production; regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of interferon-gamma production; positive regulation of tissue remodeling; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of defense response to virus by host; positive regulation of inflammatory response

0.05 mg

530 USD