antibody

Rabbit Polyclonal to Human FAP Alpha

MBS240133

0.05 mg

495 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, dog, cow

immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Rabbit Polyclonal to Human FAP Alpha

FAP Alpha

Anti-FAP Alpha Antibody (Extracellular Domain) IHC-plus; DPPIV; FAP alpha; Fibroblast activation protein; FAPA; Seprase; Human FAP Alpha

prolyl endopeptidase FAP isoform 1; Prolyl endopeptidase FAP; prolyl endopeptidase FAP; seprase; FAPalpha; dipeptidyl peptidase FAP; surface-expressed protease; post-proline cleaving enzyme; gelatine degradation protease FAP; integral membrane serine protease; serine integral membrane protease; 170 kDa melanoma membrane-bound gelatinase; fibroblast activation protein, alpha; 170 kDa melanoma membrane-bound gelatinase; Dipeptidyl peptidase FAP; Fibroblast activation protein alpha; Gelatine degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme; Surface-expressed protease;

FAP

FAP; FAP; FAPA; SIMP; DPPIV

SEPR_HUMAN

Polyclonal

Rabbit

Reactivity: Human (tested or 100% immunogen sequence identity). Predicted Reactivity: Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)

760

Human FAP. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Immunoaffinity Purified

PBS, 0.1% sodium azide.

1 mg/ml

Aliquot and store undiluted at -20 degree C or below for up to 1 year. Can be stored undiluted at 4 degree C for up to 1 month. Avoid freeze thaw cycles

Immunohistochemistry (IHC - Paraffin)

IHC-P (5 - 10 ug/ml). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.

Target: Human FAP Alpha. Immunogen Species: PAP Alpha antibody was raised against Human. Antige: Synthetic peptide. Immunogen Description: FAP Alpha antibody was raised against synthetic 17 amino acid peptide from extracellular domain of human FAP. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum (94%); Marmoset, Bat, Turkey, Chicken (88%). Epitope: Extracellular domain

Immunogen Type: Synthetic peptide. Immunogen: FAP Alpha antibody was raised against synthetic 17 amino acid peptide from extracellular domain of human FAP. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum (94%); Marmoset, Bat, Turkey, Chicken (88%). Antigen Modification: Extracellular Domain. Usage: Immunohistochemistry: MBS240133 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS240133 was determined to be 5 - 10 ug/ml.

Family: Protease. Subfamily: Serine S9

Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2 (PubMed:14751930, PubMed:16223769, PubMed:16480718, PubMed:16410248, PubMed:17381073, PubMed:18095711, PubMed:21288888, PubMed:24371721). Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vibronectin, tenascin, laminin, fibronectin, fibrin or casein (PubMed:9065413, PubMed:2172980, PubMed:7923219, PubMed:10347120, PubMed:10455171, PubMed:12376466, PubMed:16223769, PubMed:16651416, PubMed:18095711). Have also dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro (PubMed:10347120, PubMed:10593948, PubMed:16175601, PubMed:16223769, PubMed:16651416, PubMed:16410248, PubMed:17381073, PubMed:21314817, PubMed:24371721, PubMed:24717288). Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB) (PubMed:21314817). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.

16933540

NP_004451.2

NM_004460.3

Q12884

26,954 Da

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

FAP: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. Belongs to the peptidase S9B family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; Membrane protein, integral; EC 3.4.21.26; EC 3.4.14.5. Chromosomal Location of Human Ortholog: 2q23. Cellular Component: extracellular space; focal adhesion; cell surface; lamellipodium; apical part of cell; cytoplasm; plasma membrane; integral to membrane. Molecular Function: integrin binding; peptidase activity; protein dimerization activity; protein binding; protein homodimerization activity; protease binding; dipeptidyl-peptidase activity; serine-type peptidase activity; metalloendopeptidase activity; serine-type endopeptidase activity; endopeptidase activity. Biological Process: proteolysis involved in cellular protein catabolic process; endothelial cell migration; angiogenesis; cell adhesion; proteolysis; regulation of fibrinolysis

0.05 mg

495 USD