antibody

Rabbit Polyclonal to Human MAPK3 / ERK1

MBS240070

0.05 mg

495 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, cow

immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Rabbit Polyclonal to Human MAPK3 / ERK1

MAPK3 / ERK1

Anti-MAPK3 / ERK1 Antibody (Internal) IHC-plus; MAPK3; ERK-1; ERT2; HUMKER1A; HS44KDAP; MAP kinase 1; MAP kinase isoform p44; MAPK 1; MAPK 3; p44; p44MAPK; p44-MAPK; p44ERK1; Insulin-stimulated MAP2 kinase; MAP kinase 3; p44-ERK1; ERK1; PRKM3; Human MAPK3; ERK1

mitogen-activated protein kinase 3 isoform 2; Mitogen-activated protein kinase 3; mitogen-activated protein kinase 3; MAPK 1; MAP kinase isoform p44; insulin-stimulated MAP2 kinase; extracellular signal-related kinase 1; extracellular signal-regulated kinase 1; microtubule-associated protein 2 kinase; mitogen-activated protein kinase 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1

MAPK3

ERK1

MAPK3; MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; ERK1; PRKM3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK

MK03_HUMAN

Polyclonal

Rabbit

Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Pig, Rat. Predicted Reactivity: Bat, Rabbit (at least 90% immunogen sequence identity)

357

Human MAPK3 / ERK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MAPK1 (61%).

Immunoaffinity Purified

PBS, 0.1% sodium azide.

1 mg/ml

Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC - Paraffin)

IHC-P (7 ug/ml). Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.

Target Species: Human. Immunogen Description: Synthetic 18 amino acid peptide from internal region of human MAPK3 / ERK1. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Hamster, Panda, Horse, Pig (100%); Bat, Elephant, Rabbit (94%).

Immunogen Type: Synthetic peptide. Immunogen: MAPK3 / ERK1 antibody was raised against synthetic 18 amino acid peptide from internal region of human MAPK3 / ERK1. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Hamster, Panda, Horse, Pig (100%); Bat, Elephant, Rabbit (94%). Antigen Modification: Internal

Family: Protein Kinase. Subfamily: MAPK

ERK1, a MAPK -type protein kinase, is a member of the Ras-MAPK pathway. This pathway modulates the activity of transcription factors, structural proteins, and other protein kinases, leading to biological responses such as cell proliferation and differentiation. Inactive ERK1 associates with MAPK/ERK kinases (MEK) 1 and 2 in the cytosol. Upon stimulation, MEK1/2 phosphorylates and activates ERK1 causing dissociation of the MEK/ERK complex.

91718897

NP_001035145.1

NM_001040056.2

P27361

40,088 Da

AGE/RAGE Pathway (698754); ALK1 Signaling Events Pathway (137968); ARMS-mediated Activation Pathway (106466); ATF-2 Transcription Factor Network Pathway (138006); Activated TLR4 Signalling Pathway (106400); Activation Of The AP-1 Family Of Transcription Factors Pathway (160139); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adherens Junction Pathway (83070); Adherens Junction Pathway (481)

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]

ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates. Protein type: Kinase, protein; Protein kinase, CMGC; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; ERK subfamily; MAPK/ERK subfamily. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: microtubule cytoskeleton; nucleoplasm; Golgi apparatus; focal adhesion; cytoskeleton; mitochondrion; early endosome; late endosome; nuclear envelope; pseudopodium; caveola; cytosol; nucleus. Molecular Function: MAP kinase activity; protein binding; phosphotyrosine binding; ATP binding; phosphatase binding. Biological Process: axon guidance; nerve growth factor receptor signaling pathway; positive regulation of histone phosphorylation; activation of MAPKK activity; DNA damage induced protein phosphorylation; viral reproduction; apoptosis; activation of MAPK activity; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; sensory perception of pain; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; BMP signaling pathway; toll-like receptor 5 signaling pathway; response to exogenous dsRNA; regulation of transcription factor activity; small GTPase mediated signal transduction; lipopolysaccharide-mediated signaling pathway; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; transcription from RNA polymerase I promoter; cell cycle; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of stress-activated MAPK cascade; organ morphogenesis; cartilage development; Ras protein signal transduction; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; gene expression; toll-like receptor 9 signaling pathway; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; transcription initiation from RNA polymerase I promoter; blood coagulation; phosphorylation; regulation of cytoskeleton organization and biogenesis

0.05 mg

495 USD