antibody

Anti-SMARCA4 / BRG1 Antibody (C-Terminus) IHC-plus

MBS2400496

0.05 mg

660 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

Antibody

Anti-SMARCA4 / BRG1 Antibody (C-Terminus) IHC-plus

[SMARCA4 / BRG1]

[SMARCA4, ATP-dependent helicase SMARCA4, BAF190, BRG1-associated factor 190A, Brahma protein-like 1, BRG1, BRM/SWI2-related gene 1, HSNF2b, MRD16, Protein BRG-1, SNF2-like 4, SNF2LB, SNF2-BETA, SNF2B, SWI2, Nuclear protein GRB1, SNF2, SNF2L4, Transcription activator BRG1, Homeotic gene regulator, Protein brahma homolog 1, RTPS2, Sucrose nonfermenting-like 4]

[transcription activator BRG1 isoform B; Transcription activator BRG1; transcription activator BRG1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Mitotic growth and transcription activator; Protein BRG-1; Protein brahma homolog 1; SNF2-beta; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4]

[SMARCA4 / BRG1]

[SMARCA4; SMARCA4; BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A; BAF190A; BRG1; SNF2B; SNF2L4; BAF190A]

SMCA4_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat

SMARCA4 antibody is human, mouse and rat reactive. Multiple isoforms of SMARCA4 are known to exist.

Peptide Column Affinity Chromatography

PBS containing 0.02% Sodium Azide

1 mg/ml

Store at 4°C for up to 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Western Blot (WB), ELISA

IHC. IHC-Paraffin (10 ug/ml). Immunofluorescence (20 ug/ml), Western Blot (1-2 ug/ml). ELISA. (optimal dilution to be determined by the researcher). Summary: SMARCA4 antibody can be used for detection of SMARCA4 by Western Blot at 1-2 ug/ml. Antibody can also be used for Immunohistochemistry at 2 ug/ml. For Immunofluorescence start at 20 ug/ml. Antibody validated: Western Blot in human samples. Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 185kDa; Observed: 200kDa.

Immunohistochemistry (IHC)

Antigen Type: Synthetic Peptide. Immunogen Species: SMARCA4/BRG1 antibody was raised against Human. Immunogen: SMARCA4/BRG1 antibody was raised against a 19 amino acid peptide near the C-terminus of human SMARCA4. The immunogen is located within amino acids 1420-1470 of SMARCA4.

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

21071056

NP_003063.2

NM_003072.3

P51532

Chromatin Modifying Enzymes Pathway (1270434); Chromatin Organization Pathway (1270433); Direct P53 Effectors Pathway (137939); Formation Of The Beta-catenin:TCF Transactivating Complex Pathway (1269602); Glucocorticoid Receptor Regulatory Network Pathway (138014); Integrated Breast Cancer Pathway (219801); Prostate Cancer Pathway (755440); RMTs Methylate Histone Arginines Pathway (1270439); Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352); Regulation Of Retinoblastoma Protein Pathway (137916)

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

SMARCA4: a member of the SWI/SNF nucleosome remodeling complex with ATP-dependent DNA helicase activity. SWI/SNF complexes are required for mammalian development and are mutated in ~20% of all human primary tumors. Mutated in 35% (13/37) of NSCLC and 5% (1/19) of SCLC human lung cancer cell lines . A component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. Belongs to the neural progenitor-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). Component of the BAF53 complex which acetylates histone H4 and H2A within nucleosomes. Transcriptional coactivator of nuclear hormone receptors. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription, and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. SMARCA4, in consort with ZEB1, represses the transcription of E-cadherin and promotes the epithelial-to-mesenchymal transisition (EMT). SMARCA4 is required for stem cell maintenance in the mouse intestinal epithelium. Its loss inhibits aberrant Wnt-signalling and prevents tumorigenesis in the mouse small intestine. SMARCA4 mutations are the cause of a familial cancer syndrome predisposing to renal or extrarenal malignant rhabdoid tumors and to a variety of tumors of the central nervous system, including choroid plexus carcinoma, medulloblastoma, and central primitive neuroectodermal tumors. Rhabdoid tumors are the most aggressive and lethal malignancies occurring in early childhood. Belongs to the SNF2/RAD54 helicase family. The human protein includes 5 isoforms produced by alternative splicing. Protein type: Cell cycle regulation; EC 3.6.4.-; Nuclear receptor co-regulator; Tumor suppressor; EC 3.6.1.-; Helicase; Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: extracellular space; membrane; nuclear chromatin; nucleolus; nucleoplasm; nucleus; protein complex; SWI/SNF complex. Molecular Function: androgen receptor binding; ATP binding; DNA-dependent ATPase activity; helicase activity; nucleosomal DNA binding; p53 binding; protein binding; protein N-terminus binding; Tat protein binding; transcription coactivator activity; transcription corepressor activity; transcription factor binding. Biological Process: ATP-dependent chromatin remodeling; chromatin remodeling; establishment and/or maintenance of chromatin architecture; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter, mitotic; negative regulation of transcription, DNA-dependent; nervous system development; nucleosome disassembly; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of Wnt receptor signaling pathway; regulation of transcription from RNA polymerase II promoter; spermatid development; transcription, DNA-dependent. Disease: Mental Retardation, Autosomal Dominant 16; Rhabdoid Tumor Predisposition Syndrome 2

0.05 mg

660 USD