Anti-DDIT3 / CHOP Antibody IHC-plus | MyBioSource MBS2400447 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-DDIT3 / CHOP Antibody IHC-plus

catalog :

MBS2400447

quantity :

0.05 mL

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

image 2 :

Immunofluorescence analysis of A549 cell using DDIT3 antibody. Blue: DAPI for nuclear staining.

image 3 :

Western blot analysis of extracts from mouse craniofacial tissue, using DDIT3 antibody.

product information

catalog number :

MBS2400447

products type :

Antibody

products full name :

Anti-DDIT3 / CHOP Antibody IHC-plus

products short name :

[DDIT3 / CHOP]

products name syn :

[DDIT3 Antibody, CHOP10 Antibody, DDIT-3 Antibody, CHOP Antibody, GADD153 Antibody, C/EBP zeta Antibody, C/EBP-homologous protein Antibody, C/EBP-homologous protein 10 Antibody, CHOP-10 Antibody]

other names :

[DNA damage-inducible transcript 3 protein isoform 2; DNA damage-inducible transcript 3 protein; DNA damage-inducible transcript 3 protein; DNA damage inducible transcript 3; C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153]

products gene name :

[DDIT3 / CHOP]

other gene names :

[DDIT3; DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10]

uniprot entry name :

DDIT3_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse, Rat, Human

sequence length :

169

specificity :

Human DDIT3 / CHOP

purity :

Affinity purified

form :

PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.

concentration :

4.62mg/ml

storage stability :

Store at -20 degree C. Avoid freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB)

app notes :

IHC (1:50 - 1:200). IHC - Paraffin (1:200). Immunofluorescence (1:50 - 1:100). Western blot (1:500 - 1:2000)

image1 heading :

Immunohistochemistry - Paraffin (IHC)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Western Blot (WB)

image4 heading :

Western Blot (WB)

image4 description :

Western blot analysis of extracts of various cell lines.

other info1 :

Immunogen: DDIT3/CHOP antibody was raised against a synthetic peptide of human DDIT3. Immunogen Species: DDIT3/CHOP antibody was raised against Human. Antigen Type: Synthetic peptide

other info2 :

Target Species: Human. Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products description :

DDIT3 Antibody, CHOP10 Antibody, DDIT-3 Antibody, CHOP Antibody, GADD153 Antibody, C/EBP zeta Antibody, C/EBP-homologous protein Antibody, C/EBP-homologous protein 10 Antibody, CHOP-10 Antibody Description: Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes.

ncbi gi num :

21361118

ncbi acc num :

NP_004074.2

ncbi gb acc num :

NM_004083.5

uniprot acc num :

P35638

ncbi mol weight :

21,700 Da

ncbi summary :

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

uniprot summary :

CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform. Protein type: Oncoprotein; Autophagy; Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 12q13.1-q13.2. Cellular Component: cytosol; late endosome; nucleoplasm; nucleus. Molecular Function: cAMP response element binding protein binding; DNA binding; leucine zipper domain binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription corepressor activity; transcription factor activity; transcription factor binding. Biological Process: aging; blood vessel maturation; cell cycle arrest; cell redox homeostasis; cellular protein metabolic process; embryonic organ development; ER overload response; inhibition of CREB transcription factor; mRNA transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; negative regulation of myoblast differentiation; negative regulation of protein kinase B signaling cascade; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of interleukin-8 production; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; regulation of transcription in response to stress; regulation of transcription, DNA-dependent; release of sequestered calcium ion into cytosol; response to amphetamine; response to DNA damage stimulus; response to drug; response to hydrogen peroxide; response to nutrient; response to starvation; response to unfolded protein; unfolded protein response; Wnt receptor signaling pathway. Disease: Myxoid Liposarcoma

size1 :

0.05 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage