Anti-CD20 Antibody IHC-plus | MyBioSource MBS2400340 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-CD20 Antibody IHC-plus

catalog :

MBS2400340

quantity :

0.05 mg

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Mouse Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)

product information

catalog number :

MBS2400340

products type :

Antibody

products full name :

Anti-CD20 Antibody IHC-plus

products short name :

[CD20]

products name syn :

[Rabbit Polyclonal (IgG) to Mouse CD20; Mouse CD20; MS4A1; B-lymphocyte antigen CD20; B1; Bp35; CD20; CD20 antigen; CD20 receptor; LEU-16; CVID5]

other names :

[B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; membrane spanning 4-domains A1; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD_antigen: CD20]

products gene name :

[CD20]

products gene name syn :

[MS4A1]

other gene names :

[MS4A1; MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16; CD20]

uniprot entry name :

CD20_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

rabbit

reactivity :

Mouse

sequence length :

297

specificity :

Mouse CD20

purity :

Caprylic acid and ammonium sulfate precipitation

form :

PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.

concentration :

1 mg/ml

storage stability :

Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)

app notes :

IHC-P (5 ug/ml) . Optimal dilution to be determined by the researcher.

image1 heading :

Immunohistochemistry - Paraffin (IHC)

other info1 :

Immunogen: CD20 antibody was raised against recombinant mouse B-lymphocyte antigen CD20 protein. Immunogen Species: CD20 antibody was raised against Mouse. Antigen Type: Recombinant protein. Target: CD20

other info2 :

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip. Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening. Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.

products description :

MS4A1 Antibody, B-lymphocyte antigen CD20 Antibody, B1 Antibody, Bp35 Antibody, CD20 Antibody, CD20 antigen Antibody, CD20 receptor Antibody, LEU-16 Antibody, CVID5 Antibody Description: CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members.

ncbi gi num :

23110989

ncbi acc num :

NP_690605.1

ncbi gb acc num :

NM_152866.2

uniprot acc num :

P11836

ncbi pathways :

Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489)

ncbi summary :

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

uniprot summary :

MS4A1: This protein may be involved in the regulation of B-cell activation and proliferation. Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the MS4A family. Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell surface. Chromosomal Location of Human Ortholog: 11q12. Cellular Component: external side of plasma membrane; extracellular space; integral to plasma membrane. Molecular Function: epidermal growth factor receptor binding; protein binding. Biological Process: B cell proliferation; humoral immune response. Disease: Immunodeficiency, Common Variable, 5

size1 :

0.05 mg

price1 :

530 USD

more info or order :

MyBioSource product webpage