NID1, Enactin, Nidogen 1, NID, Nidogen, Entactin, NID-1, Nidogen (enactin), Nidogen-1 | MyBioSource MBS2400150 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

NID1, Enactin, Nidogen 1, NID, Nidogen, Entactin, NID-1, Nidogen (enactin), Nidogen-1

catalog :

MBS2400150

quantity :

0.05 mL

price :

530 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

image

image 1 :

Anti-NID1 / Entactin / Nidogen antibody IHC staining of human placenta, endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

image 2 :

Western blot of extracts from K562 cells, using NID1 Antibody. The lane on the right is treated with the synthesized peptide.

product information

catalog number :

MBS2400150

products type :

Antibody

products full name :

NID1, Enactin, Nidogen 1, NID, Nidogen, Entactin, NID-1, Nidogen (enactin), Nidogen-1

products short name :

[NID1 / Entactin / Nidogen-1]

products name syn :

[Rabbit Polyclonal (IgG) to Human NID1 / Entactin / Nidogen-1; Human NID1 / Entactin / Nidogen-1; Enactin; Nidogen 1; NID; Nidogen; Entactin; NID-1; Nidogen (enactin); Nidogen-1]

other names :

[nidogen-1; Nidogen-1; nidogen-1; nidogen 1; Entactin]

products gene name :

[NID1]

other gene names :

[NID1; NID1; NID; NID; NID-1]

uniprot entry name :

NID1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Mouse, Rat, Human

sequence length :

1247

specificity :

NID1 Antibody detects endogenous levels of total NID1 protein.

purity :

Immunoaffinity purified

form :

PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol

concentration :

1 mg/ml

storage stability :

Store at -20 degree C for up to one year.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)

app notes :

ELISA (1:20000). IHC-Paraffin (1:50). WB (1:500 - 1:1000). Optimal dilution to be determined by researcher

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

other info1 :

Immunogen: NID1 / Entactin / Nidogen-1 antibody was raised against synthetic peptide from human NID1 (aa219-268). Immunogen Species: NID1 / Entactin / Nidogen-1 antibody was raised against Human. Epitope: aa219-268. Target: NID1/ Entactin/ Nidogen-1. Antigen Type: Synthetic Peptide.

other info2 :

Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application. Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:. Tissue Preparation: . Formalin fixation and embedding in paraffin wax. Tissue Sectioning: . Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. Deparaffinization: . Wash dry slides in 3 changes of xylene - 5 minutes each @ RT. Rehydration: . Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT. Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT. Wash slides in 1 change of 80% alcohol - 3 minutes @ RT. Rinse slides in gentle running distilled water - 5 minutes @ RT. Antigen retrieval: . Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Remove from heat and let stand at room temperature in buffer - 20 minutes. Rinse in 1X TBS with Tween (TBST) -1 minute @ RT. Immunostaining: . (Do not allow tissues to dry at any time during the staining procedure) . Apply a universal protein block - 20 minutes @ RT. Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT. Rinse slides in 1 X TBST - 1 minute @ RT. Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase streptavidin - 30 minutes @ RT. Rinse slides in 1X TBST -1 minute @ RT. Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT. Wash slides in distilled water - 1 minute @ RT. Dehydrate: . (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) . Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT. Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT. Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT. Wash slides in 3 changes of xylene - 1 minute each @ RT. Apply coverslip

products description :

NID1 Antibody, Enactin Antibody, Nidogen 1 Antibody, NID Antibody, Nidogen Antibody, Entactin Antibody, NID-1 Antibody, Nidogen (enactin) Antibody, Nidogen-1 Antibody Description: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

ncbi gi num :

115298674

ncbi acc num :

NP_002499.2

ncbi gb acc num :

NM_002508.2

uniprot acc num :

P14543

ncbi mol weight :

122,017 Da

ncbi pathways :

Degradation Of The Extracellular Matrix Pathway (1270257); Extracellular Matrix Organization Pathway (1270244); Laminin Interactions Pathway (1270253)

ncbi summary :

This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq, Jul 2008]

uniprot summary :

Nidogen-1: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell- extracellular matrix interactions. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q43. Cellular Component: basal lamina; basement membrane; extracellular matrix; extracellular region. Molecular Function: calcium ion binding; collagen binding; laminin binding; laminin-1 binding; proteoglycan binding. Biological Process: cell-matrix adhesion; extracellular matrix disassembly; extracellular matrix organization and biogenesis; glomerular basement membrane development

size1 :

0.05 mL

price1 :

530 USD

more info or order :

MyBioSource product webpage