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MAGIC RED CATHEPSIN B KIT

MBS239215

25 Tests

340 USD

Kits

MAGIC RED CATHEPSIN B KIT

CATHEPSIN B

cathepsin B; Cathepsin B; cathepsin B; BCSB; cathepsin B<; BCSBCleaved into the following 2 chains:Cathepsin B light chain; Cathepsin B heavy chain

CTSB; CTSB

CATB_BOVIN

335

Kit

Store the unopened kit (and each unopened component) at 4 degree C until the expiration date. Protect the MR-(RR)2 reagent from light at all times. Once reconstituted, the 260X MR-(RR)2 stock should be stored at -20 degree C protected from light. Shelf Life: The 260X MR-(RR)2 reconstituted stock is stable for up to 6 months and may be thawed twice during that time.

Immunofluorescence (IF)

Reagents In The Kits: Magic Red MR-(RR)2 Reagent - lyophilized . Hoechst 33342 Stain, 1 mL. Acridine orange (AO), 0.5 mL

Description: The Magic Red Cathepsin B kit uses a quick and easy method to analyze intracellular Cathepsin B protease activity in whole living cells. Test Principle: Cathepsin Magic Red kits measure cathepsin B protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product. The Magic Red reagent contains a cathepsin B target sequence peptide (RR)2 linked to a red (Cresyl Violet) fluorescent probe. The Cathepsin Magic Red kits are suitable for cells in suspension, adherent cells, thin frozen tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits. This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.

27806671

NP_776456.1

NM_174031.2

36,661 Da

Antigen Processing And Presentation Pathway (84313); Antigen Processing And Presentation Pathway (485); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1120044); Collagen Degradation Pathway (1120056); Collagen Formation Pathway (1120042); Degradation Of The Extracellular Matrix Pathway (1120054); Extracellular Matrix Organization Pathway (1120041); Immune System Pathway (1119286); Innate Immune System Pathway (1119319); Lysosome Pathway (98762)

25 Tests

340 USD