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MAGIC RED CATHEPSIN L KIT

MBS239203

100 Tests

725 USD

Kits

MAGIC RED CATHEPSIN L KIT

CATHEPSIN L

cathepsin L, partial; Cathepsin L; Cathepsin L

CatL

Q7Z0G8_METEN

306

Kit

Store the unopened kit (and each unopened component) at 4 degree C until the expiration date. Protect the MR-(FR)2 reagent from light at all times. Once reconstituted, the 260X MR-(FR)2 stock should be stored at -20 degree C protected from light. Shelf Life: The 260X MR-(FR)2 reconstituted stock is stable for up to 6 months and may be thawed twice during that time.

Immunofluorescence (IF)

Reagents In The Kits: Magic Red MR-(FR)2 Reagent - lyophilized. Hoechst 33342 Stain, 1 mL. Acridine orange (AO), 0.5 mL

Description: The Magic Red Cathepsin L kit uses a quick and easy method to analyze intracellular Cathepsin L protease activity in whole living cells. Test Principle: Cathepsin Magic Red kits measure Cathepsin L protease activity by detecting active cathepsins in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a cell-permeable and non-cytotoxic reagent which is cleaved in the presence of cathepsins to produce a fluorescent product. The Magic Red reagent contains a Cathepsin L target sequence peptide (FR)2 linked to a red (Cresyl Violet) fluorescent probe. The Cathepsin Magic Red kits are suitable for cells in suspension, adherent cells, thin frozen tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect and yeast. Different cell types, e.g. Jurkat, HL-60, THP-1, fibroblasts, UMUC-3, MCF-7 and U937 cells have also been successfully studied with these kits. This kit can be used in fluorescence microscopy or with fluorescence plate readers using black microtitre plates.

32394730

AAM96001.1

34,176 Da

100 Tests

725 USD