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MITOCHONDRIAL MEMBRANE POTENTIAL KIT

MBS239179

500 Tests

570 USD

Kits

MITOCHONDRIAL MEMBRANE POTENTIAL KIT

MITOCHONDRIAL MEMBRANE POTENTIAL MitoPT TMRE KIT

Kit

Store the unopened kit (and each unopened component) at -20 degree C until the expiration date. Protect the MitoPTTM TMRE reagent from light at all times. Once reconstituted, the 1mM MitoPTTM TMRE stock should be stored at -20 degree C protected from light. Shelf Life: The 1mM MitoPTTM TMRE reconstituted stock is stable for up to 12 months and may be thawed three times during that time.

Flow cytometry (FC/FACS), Immunofluorescence (IF)

Reagents In The Kits: MitoPTTM TMRE Reagent - lyophilized - to make up 1mM stock, 10X Assay Buffer, 125mL, 600uL vial 50mM Carbonylcyanide m - chlorophenylhydrazone (CCCP) concentrate in DMSO.

Description: The MitoPTTM TMRE kit uses a quick and easy staining method to clearly differentiate between non-apoptotic and apoptotic cells through mitochondrial functionality. Test Principle: An early indication of apoptosis involves a collapse in the electrochemical gradient across the mitochondrial membrane. Loss of mitochondrial membrane potential can be detected by a unique fluorescent cationic dye known as TMRE (tetramethylrhodamine ethyl ester), that has been incorporated into the MitoPTTM TMRE kit. The MitoPTTM TMRE reagent easily penetrates cells and enters the mitochondria. It aggregates in the mitochondria of non-apoptotic cells and fluoresces bright orange/red, whilst in apoptotic cells it diffuses throughout the cell. Once dispersed, the reagent assumes a monomeric form and exhibits a reduced orange/red fluorescence level. This allows an easy distinction between apoptotic and non-apoptotic fluorescent cells which can be read with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.

500 Tests

570 USD