protein

NATIVE HEPATITIS A VIRUS

MBS239140

1 mL

235 USD

Antigen

NATIVE HEPATITIS A VIRUS

HEPATITIS A VIRUS

Inactivated Pathogen. Inactivated Hepatitis A virus - liquid.

Total protein concentration 0.30 mg/ml

Store at -70 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Shelf Life: 18 months from date of despatch.

ELISA (EIA)

Source: Hepatitis A virus, strain HM-175, cultured in FRhK 4, an established cell line from foetal Rhesus monkey kidney. Preparation: Cells infected with Hepatitis A virus are collected by centrifugation. Pelleted cells are resuspended in glycine buffer and disrupted to release viral particles. Cellular debris is removed by centrifugation and the antigen preparation is further purified by extraction with freon. The antigen preparation is inactivated by incubating with formaldehyde for 96 hours. Instructions For Use: This product should be sonicated immediately before use to ensure the preparation is uniform. The product may be used in a variety of immunoassay formats or may be further purified to meet the requirements of a particular assay format.

Buffer Solution: Glycine buffered saline . Activity: This product has been rendered inactive by standard procedures. However this material should still be handled as infectious and should be disposed of appropriately. Target Species: Viral

Native Hepatitis Virus Hepatitis A is a wide-spread infectious disease, resulting from infection of the liver by the Hepatitis A virus (HAV). Hepatitis A infection causes jaundice, fever, anorexia, nausea, vomiting, headache and fatigue. Some cases may be fatal. The virus is mainly transmitted by the faecal-oral route via contaminated food or drinking water. The HAV is a picornavirus that replicates slowly without noticeable interference to host cell macro-molecular synthesis and, consequently, without apparent cytopathic effect. The liver pathology observed is likely to be immune-mediated. Hepatitis A virus is a non-enveloped, icosahedral virion with a single stranded RNA genome. Only one serotype of HAV is known and it does not cross-react with Hepatitis B virus. The virus contains four proteins VP1, VP2, VP3 and VP4. This product contains a high concentration of viral antigen, predominantly in the form of whole virions. The preparation also contains a low concentration of formaldehyde with some residual host cellular material.

1 mL

235 USD