product summary
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company name :
MyBioSource
product type :
other
product name :
TMB CORE+
catalog :
MBS239112
quantity :
100 mL
price :
220 USD
more info or order :
product information
catalog number :
MBS239112
products type :
Accessory Reagent
products full name :
TMB CORE+
products short name :
TMB CORE+
products gene name :
TMB
form :
Ready To Use. Ready to use TMB solution - liquid
storage stability :
Store at 4 degree C. DO NOT FREEZE. This product is photosensitive and should be protected from light. Avoid exposure to heat and contamination with metal ions or peroxidase. Store in bottles made of High Density Polyethylene (HDPE). Shelf Life: 48 months from date of despatch.
tested application :
ELISA (EIA)
app notes :
ELISA: Minimum Dilution: Neat
other info1 :
Intended Use: This item is a high performance TMB (3,3´, 5, 5´- tetramethylbenzidine) solution, recommended for use in ELISA as a substrate for horseradish peroxidase (HRP). This item contains TMB, substrate buffer and hydrogen peroxide in a safe, ready to use solution. The activity of TMB has been optimised to enable increased sensitivity, minimal background and rapid development. This item produces a deep blue colour during the enzymatic degradation of H2O2 by horseradish Peroxidase. The reaction may be stopped with 0.2M sulphuric acid, resulting in a yellow colour read at 450nm. Instructions For Use: 1. It is recommended that 100ul of this item TMB substrate is used per microtiter well. Pour the desired amount of substrate into a sealed container and allow it to reach room termperature in the dark. 2. Add 100ul substrate solution per microtiter well. 3. Allow development of the substrate solution. Time of development is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation e.g. wrapped in tinfoil. 4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour. N.B. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of This item is not recommended).
size1 :
100 mL
price1 :
220 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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