catalog number :
MBS238277
products type :
Accessory Reagent
products full name :
10x ELISA WASH BUFFER
products short name :
10x ELISA WASH BUFFER
form :
Concentrate. 10 x concentration - liquid
storage stability :
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil. Shelf Life: 24 months from date of despatch.
tested application :
ELISA (EIA)
other info1 :
Perservative Stabilisers: 1.0% 2-chloroacetamide. Intended Use: ELISA wash buffer was developed as a high performance washing solution for use in all ELISA formats. The optimised formulation of pH stabilizers, salts and detergents increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal. This item is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase. Instructions For Use: 1. Dilute the required volume of this item 1:10 with deionised water (1 part This item to 9 parts water) and mix. 2. Use at each ELISA wash step for a total of 2-4 washes. Fill each well with the diluted wash buffer (approximately 400ul), taking care not to submerge the plate as this can cross-contaminate the wells. The buffer may be dispensed manually or by an automated system.3. Between washes, aspirate or dump the buffer. After the final wash blot the plate on paper towels to remove any excess liquid.
other info2 :
Buffer Solution: Phosphate buffered saline