protein

RECOMBINANT HUMAN TIMP-1

MBS232506

0.01 mg

440 USD

Recombinant Protein

RECOMBINANT HUMAN TIMP-1

TIMP-1

metalloproteinase inhibitor 1; Metalloproteinase inhibitor 1; metalloproteinase inhibitor 1; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1; TIMP metallopeptidase inhibitor 1; Erythroid-potentiating activity; EPA; Fibroblast collagenase inhibitor; Collagenase inhibitor; Tissue inhibitor of metalloproteinases 1; TIMP-1

TIMP-1

TIMP1; TIMP1; EPA; EPO; HCI; CLGI; TIMP; CLGI; TIMP; EPA; Collagenase inhibitor; TIMP-1

TIMP1_HUMAN

207

>95% by SDS PAGE and HPLC analysis

Purified recombinant protein - lyophilised

Approximate Protein Concentration: 0.1mg/ml after reconstitution

Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 3 months from date of reconstitution.

ELISA (EIA), Functional Assays (FN), Western Blot (WB)

ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;. Functional Assays: Minimum Dilution: 5.0; Maximum Dilution: 10.0ng/ml;. Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane

Reconstitution: Reconstitute with 100ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% BSa is recommended. Preparation: Purified recombinant TIMP-1 expressed in E Coli

Buffer Solution: 10mM Sodium Phosphate, pH7.5. Activity: TIMP-1 activity was measured by its ability to inhibit human MMP-1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP-1 concentration of approximately 0.5 mug/ml and MMP-1 concentration of 1.6 mug/ml. Target Species: Human

TIMP-1 (Tissue Inhibitor of Metalloproteinase -1), is a secreted glycoprotein and member of the protease inhibitor I35 (TIMP) family, which is expressed by a wide range of cells, including microvascular endothelial cells, smooth muscle cells, mesangial cells, and some fibroblasts. TIMP-1 acts as a natural extracellular inhibitor of MMPs (matrix metalloproteinases), a group of peptidases involved in degradation of the extracellular matrix, and is also involved in erythropoiesis, through the simulation of the proliferation/differentiation of erythroid progenitors. Studies have shown that a correlation between localized concentrations of TIMP-1 and TIMP-3, may determine cell fate, and that increased expression of TIMP-1, may act as an inhibitor/suppressor of invasive cell growth.

4507509

NP_003245.1

NM_003254.2

P01033

20.6kD (184 amino acid residues)

Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); HIF-1 Signaling Pathway (695200); Hemostasis Pathway (106028); IL6-mediated Signaling Events Pathway (137932); Matrix Metalloproteinases Pathway (198900); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]

TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family. Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: Xp11.3-p11.23. Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region; basement membrane. Molecular Function: metalloendopeptidase inhibitor activity; protein binding; growth factor activity; protease binding; metal ion binding; cytokine activity. Biological Process: response to peptide hormone stimulus; platelet activation; extracellular matrix organization and biogenesis; negative regulation of metalloenzyme activity; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; platelet degranulation; response to cytokine stimulus; positive regulation of cell proliferation; blood coagulation; negative regulation of apoptosis; aging

0.01 mg

440 USD