protein

RECOMBINANT HUMAN DEFENSIN ALPHA 1

MBS232451

0.02 mg

440 USD

Recombinant Protein

RECOMBINANT HUMAN DEFENSIN ALPHA 1

DEFENSIN ALPHA 1

neutrophil defensin 1 isoform 2 preproprotein; Neutrophil defensin 1; neutrophil defensin 1; defensin, alpha 1B; Defensin, alpha 1; HNP-1; HP-1; HP1

DEFA1B; DEFA1; HP1; HP-1; HNP-1; DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2

DEF1_HUMAN

94

>98% by SDS PAGE and HPLC analysis

Purified recombinant protein - lyophilised

Approximate Protein Concentration: 0.1mg/ml after reconstitution

Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 3 months from date of reconstitution.

ELISA (EIA), Functional Assays (FN), Western Blot (WB)

ELISA: Recombinant human defensin-alpha-1 may be used as a standard for ELISA applications with either a purified anti human defensin-alpha-1 antibody or a biotinylated anti human defensin-alpha-1 antibody. Western Blot: Recombinant human defensin-alpha-1 may be used as a positive control for Western Blotting applications with either a purifed human defensin-alpha-1 antibody or a biotinylated human defensin-alpha-1 antibody. ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well. Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane

Reconstitution: Reconstitute with 0.2ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. Preparation: Purified recombinant defensin-alpha-1 expressed in E Coli

Activity: Determined by its ability to chemoattract immature dendritic cells using a concentration of 1.0-10.0 ng/ml. Target Species: Human

Recombinant Human Defensin-alpha-1 is a 3.4kDa peptide comprised of 30 amino acid residues corresponding to full-length mature human defenstin alpha 1. Defensin-alpha-1, otherwise known as HNP-1, is a secreted cationic antimicrobial peptide and member of the alpha-defensin family, which plays an important role in the innate immune response against microbial infections.

124248516

NP_001035965.1

NM_001042500.1

P59665

3.4kD (30 amino acid residues)

Alpha-defensins Pathway 530758!!Cellular Roles Of Anthrax Toxin Pathway 138076!!Defensins Pathway 530757!!Immune System Pathway 106386!!Innate Immune System Pathway 106387

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

0.02 mg

440 USD