catalog number :
MBS232387
products type :
Recombinant Protein
products full name :
RECOMBINANT RAT CNTF
products short name :
CNTF
other names :
ciliary neurotrophic factor; Ciliary neurotrophic factor; ciliary neurotrophic factor; ciliary neurotropic factor; ciliary neurotrophic factor; N/A
products gene name :
CNTF
other gene names :
Cntf; Cntf; CNTF
uniprot entry name :
CNTF_RAT
purity :
>98% by SDS PAGE and HPLC analysis
form :
Purified recombinant protein - lyophilised
concentration :
Approximate Protein Concentration: 1.0 mg/ml
storage stability :
Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 3 months from date of reconstitution.
tested application :
ELISA (EIA), Functional Assays (FN), Western Blot (WB)
app notes :
ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;. Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
other info1 :
Reconstitution: Centrifuge vial prior to opening. Reconstitute with 25ul of water.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. DO NOT VORTEX. Preparation: Sterile filtered through a 0.2 micron filter, lyophilised from 5mM Sodium Acetate pH 6.5
other info2 :
Activity: Determined by it's ability to stimulate the proliferation of human TF-1 cells using a concentration range of 25.0 - 35.0 ng/ml. Target Species: Rat
products description :
Recombinant rat ciliary neurotrophic factor prepared from E Coli.Ciliary neutrophic factor (CNTF) is a neuroregulatory cytokine which is found primarily in peripheral nerve tissue. CNTF promotes survival and differentiation of neurons and other neuronal cell types.
ncbi acc num :
NP_037298.1
ncbi gb acc num :
NM_013166.1
ncbi mol weight :
Protein Molecular Weight: 22.7 kDa (199 amino acid sequence)
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway 83443!!Cytokine-cytokine Receptor Interaction Pathway 460!!Delta-Notch Signaling Pathway 198480!!Jak-STAT Signaling Pathway 83469!!Jak-STAT Signaling Pathway 488
ncbi summary :
a cytokine involved in neuronal degeneration and adipocyte gene expression [RGD, Feb 2006]