catalog number :
MBS232385
products type :
Recombinant Protein
products full name :
RECOMBINANT RAT INTERLEUKIN-1 BETA
products short name :
IL-1 BETA
other names :
Interleukin-1 beta; Interleukin-1 beta; interleukin-1 beta; IL-1 beta; interleukin 1 beta; N/A
other gene names :
Il1b; Il1b; IL-1 beta
uniprot entry name :
IL1B_RAT
purity :
>98% by SDS PAGE and HPLC analysis
form :
Purified recombinant protein - lyophilised
concentration :
Total protein concentration 0.1 mg/ml after reconstitution
storage stability :
Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 3 months from date of reconstitution.
tested application :
ELISA (EIA), Functional Assays (FN), Western Blot (WB)
app notes :
ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;. Functional Assays: Minimum Dilution: 0.1; Maximum Dilution: 10ng/ml;. Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
other info1 :
Reconstitution: Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. Preparation: Recombinant protein expressed in Escherichia coli.
other info2 :
Buffer Solution: 10mM Sodium Acetate pH6.5. Activity: This item has a specific activity of > 1 x 107 units/mg as determined by dose-dependent stimulation of proliferation of murine D10S cells. Target Species: Rat
products description :
MBS232385 is an E Coli-derived recombinant protein corresponding to full-length mature rat interleukin 1 beta (IL-1b), an immunomodulary cytokine that mediates a wide range of immune responses.
ncbi mol weight :
Protein Molecular Weight: 17.3kD (153 Amino acid sequence)
ncbi pathways :
African Trypanosomiasis Pathway 194377!!African Trypanosomiasis Pathway 194323!!Alzheimer's Disease Pathway 83489!!Alzheimer's Disease Pathway 509!!Amoebiasis Pathway 167311!!Amoebiasis Pathway 167191!!Apoptosis Pathway 83452!!Apoptosis Pathway 470!!Chagas Disease (American Trypanosomiasis) Pathway 147806!!Chagas Disease (American Trypanosomiasis) Pathway 147795
ncbi summary :
an inflammatory cytokine [RGD, Feb 2006]