protein

RECOMBINANT MOUSE INSULIN-LIKE GROWTH FACTOR I

MBS232345

0.01 mg

375 USD

Recombinant Protein

RECOMBINANT MOUSE INSULIN-LIKE GROWTH FACTOR I

IGF-I

insulin-like growth factor I isoform 4 preproprotein; Insulin-like growth factor I; insulin-like growth factor I; somatomedin; insulin-like growth factor 1; Somatomedin

Igf1; Igf1; Igf-1; Igf-I; C730016P09Rik; Igf-1; IGF-I

IGF1_MOUSE

153

>97% by SDS PAGE and HPLC

Purified recombinant protein - lyophilised

Approximate Protein Concentration: 1.0 mg/ml after reconstitution

Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 3 months from date of reconstitution.

ELISA (EIA), Functional Assays (FN), Western Blot (WB)

ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;. Functional Assays: Minimum Dilution: 0.1; Maximum Dilution: 1.0ng/ml;. Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane

Reconstitution: Reconstitute with 10 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide.

Target Species: Mouse

MBS232345 is an E Coli-derived recombinant protein corresponding to full-length mature murine insulin-like growth factor 1 (IGF-1), a 70 amino acid peptide that is structurally and functionally related to insulin but with higher growth-promoting activities. Recombinant mouse IGF-1 has an ED50 of

163659894

NP_001104745.1

NM_001111275.1

P05017

Protein Molecular Weight: 7.6kD (70 amino acid sequence)

AMPK Signaling Pathway (989651); AMPK Signaling Pathway (992181); Adipogenesis Pathway (198299); Aldosterone-regulated Sodium Reabsorption Pathway (130627); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Androgen Receptor Signaling Pathway (198319); Apoptosis Pathway (198339); Dilated Cardiomyopathy Pathway (121495); Dilated Cardiomyopathy Pathway (121285); Endochondral Ossification Pathway (198336)

IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted. Cellular Component: extracellular space; cell soma; extracellular region; intracellular. Molecular Function: integrin binding; insulin-like growth factor receptor binding; protein serine/threonine kinase activator activity; protein binding; growth factor activity; hormone activity; steroid binding; insulin receptor binding. Biological Process: positive regulation of transcription, DNA-dependent; regulation of protein metabolic process; chondroitin sulfate proteoglycan biosynthetic process; exocrine pancreas development; water homeostasis; positive regulation of glucose import; positive regulation of fibroblast proliferation; proteoglycan biosynthetic process; inner ear development; positive regulation of DNA binding; muscle hypertrophy; nervous system development; positive regulation of protein import into nucleus, translocation; positive regulation of mitosis; regulation of establishment and/or maintenance of cell polarity; regulation of calcium ion transport; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; insulin-like growth factor receptor signaling pathway; response to heat; regulation of gene expression; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of insulin-like growth factor receptor signaling pathway; myoblast proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of glycogen biosynthetic process; negative regulation of caspase activity; detection of mechanical stimulus involved in sensory perception; positive regulation of smooth muscle cell migration; positive regulation of activated T cell proliferation; regulation of translation; negative regulation of cell proliferation; glial cell differentiation; positive regulation of MAPKKK cascade; mammary gland development; positive regulation of cell proliferation; regulation of steroid hormone receptor signaling pathway; positive regulation of granule cell precursor proliferation; regulation of protein amino acid phosphorylation; phosphoinositide-mediated signaling; multicellular organism growth; myotube cell development; satellite cell compartment self-renewal involved in skeletal muscle regeneration; memory; regulation of cell proliferation; myoblast differentiation; cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of osteoblast differentiation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of glycolysis; blood vessel remodeling; positive regulation of Ras protein signal transduction; cell development; positive regulation of DNA replication; lung development

0.01 mg

375 USD